母爱剥夺雄性大鼠成年后的行为特征

Behavioral characteristics of adult male rats after experiencing neonatal maternal deprivation

目的研究经历了新生期母爱剥夺应激的雄性大鼠成年以后的行为特征。方法在SD大鼠出生后的第1天，随机分为实验组（16只）和对照组（14只）。实验组在出生后第1～14天，每天接受6h（9：00～15：00）的母爱剥夺，对照组不接受任何处理。出生后第21天断乳并分笼饲养直至第10周。采用Morris水迷宫实验、高架十字迷宫、旷场实验对2组大鼠的空间学习记忆能力、焦虑水平、探索行为进行评定。结果Morris水迷宫实验中，实验组大鼠逃避潜伏期的时间[（83．66±22．91）s]明显长于对照组[（56．98±20．89）S，t=3．32，P〈0．05]，穿越平台的次数实验组[（2．56±1．55）次]明显少于对照组[（4．21±1．58）s，t=2．89，P〈0．05]，在目标象限停留的时间实验组[（25±9．52）s]少于对照组[（32．87±8．27）S，t=2．39，P〈0．05]；旷场实验中，实验组大鼠爬行的总距离[（17．41±2．24）m]明显短于对照组[（22．86±2．89）m，t=5．81，P〈0．01]，而在中央格停留的时间2组之间的差异无显著性意义（t=2．38，P〉0．05）；高架十字迷宫实验中，实验组在开臂中停留的时间长于对照组大鼠（u=2．35，P〈0．05），进入开臂的次数2组之间差异无显著性（u=-0．99，P〉0．05），进入闭臂次数实验组明显多于对照组大鼠（u=2．351，P〈0．05）。结论经历了新生期母爱剥夺的雄性大鼠成年以后的行为能力在多方面受到了不同程度的损害。

Objective This paper is to study the behavioral characterization of adult male rats after experiencing neonatal maternai deprivation. Methods A maternal deprivation model was used. The male pups were random]y assigned to maternal deprivation group （ n= 16） and control group （ n= 14） on postnatal day 1. Maternal deprivation rats were removed from their mother for 6 hours per day（9：00 - 15： 00）. When the pups were 10 weeks old, behavioral tests including Morris water maze, an open field test and an elevated plus maze test were cartied out to evaluate their spatial learning and memory, the exploration ability in new environment and the level of anxiety. Results Two groups were significantly different in mean latency to reach the platform [ maternal deprivation group（83.66 ± 22.91 ） ; control group（56.98 ±20.89） ] in the Morris water maze experiment. Open-field test revealed that the exploratory ability decreased compared to the controls [ maternal deprivation group （ 17.41 ± 2.24） ;control group （22.86 ± 2.89 ） ]. The elevated plus maze experiment show that maternal deprivation group spent a greater amount of time in the open arms （u = 2.35, P 〈 0.05 ）. Conclusion The adult male rats experienced neonatal maternal separation showed impairment in some behaviors.