樟脑气味嗅觉条件刺激诱发小鼠条件反射性抗体增强的实验研究

Conditioned enhancement of antibody response against influenza virus hemagglutinin using camphor odor as conditioned stimulus in mice

目的对流感疫苗（流感病毒血凝素表面抗原）的接种，观察条件反射性免疫反应。方法采用36只BALB／c小鼠肌肉注射流感疫苗抗原（3μg／每只动物）为非条件性刺激，与樟脑气味嗅觉条件刺激一次性结合。第6周末再次给予条件刺激，观察条件反射性流感疫苗的抗体反应。结果条件刺激组在一次性条件刺激后，抗流感疫苗的抗体水平OD值增高（第9周0．68±0．06；第10周0．60±0．06），与非条件刺激组（第9周0．53±0．06；第10周0．48±0．04）比较差异有显著性（P〈0．01）。与条件训练对照组和非条件训练对照组比较差异有显著性（P〈0．05）。结论经过一次樟脑气味嗅觉条件刺激与流感疫苗非条件刺激的结合训练后，单独条件刺激能够诱导动物出现条件反射性抗体增强反应。

Objective To observe conditioned enhancement of antibody production against influenza vaccine. Methods 36 female BALB/c mice were injected with 3μg/mouse influenza virus hemagglutinin （HA） as the unconditioned stimulus （UCS） ,and camphor odor was served as the conditioned stimulus （CS）. After a CS/ UCS pairing was made, animals were re-exposed to the CS at Weeks 6. Results Through one conditioned stimulus,the optical density of anti-HA antibody of the conditioned group （Weeks 9：0.68 ±0. 06; Weeks 10：0.60 ± 0.06 ） was significantly increased compared with the unconditioned group （ Weeks 9：0.53 ± 0.06 ; Weeks 10：0.48 ±0, 04） （P〈0. O1 ）. The level of anti-HA antibody of the conditioned group was also significantly greater than other controlled groups （P 〈 0.05 ）. Conclusion Through a single exposure to camphor odor which was paired with immunization of influenza virus HA in a single trial learning protocol, a significant conditioned anti-HA IgG production occurred.