摘要
目的探讨金属蛋白酶解离素28(ADAM28)反义核酸对人牙囊细胞(HDFC)内ADAM28蛋白表达的影响。方法应用反义核酸技术,设计并合成与ADAM28 mRNA特异序列互补的AS-ODN和正义对照S-ODN转染HDFC后,通过免疫细胞化学、RT-PCR和Western blot检测细胞中ADAM28的表达差异。结果ADAM28 AS-ODN转染HDFC后,在转录、翻译和蛋白水平细胞内ADAM28的表达均明显减弱,统计学分析有显著性差异(P<0.01)。结论ADAM28 AS-ODN可有效封闭HDFC内ADAM28的表达。
Objective To investigate tile effects of ADAM28 AS-ODN on the expressions of ADAM28 protein in HDFC. Methods The sequences specifically targeting at ADAM28 mRNA were designed and synthesized as ADAM28 AS-ODN and S-ODN by antisense oligonu- cleofides technique and ADAM28 AS-ODN and S-ODN were transfected into HDFC. The expression differences of ADAM28 in HDFC were de- tected by immunocytochemistry, RT-PCR and Western blot assays. Results The expressions of ADAM28 in HDFC were weakened significantly in transcription,translation and protein level after AS-ODN was transfected into HDFC. There were significant differences in statistical analyses（ P 〈 0.01 ）. Conclusion ADAM28 AS-ODN could effectively block the expressions of ADAM28 in HDFC.
出处
《口腔医学》
CAS
2009年第5期230-234,共5页
Stomatology
基金
国家自然科学基金资助课题(30572046)
