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免疫荧光染色检测克隆牛X染色体组蛋白H3Lys4二甲基化修饰 被引量：1

Immunofluorescence patterns of histone H3 lysine 4 dimethylation on X chromosomes of different cloned cattle
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摘要 利用免疫荧光染色,检测胎儿成纤维细胞(FFB)和输卵管上皮细胞(FOV)来源的克隆牛X中期染色体组蛋白H3K4m2修饰状况。结果发现,这两种细胞系来源的克隆牛耳组织细胞系X染色体H3K4m2修饰与供体核FFB细胞系和常规繁殖牛耳组织细胞系的修饰基本一致,而与供体核FOV细胞系有较大差异。 Using immunofluorescence staining, the patterns of histone H3 K4m2 of metaphase X chromosomes were measured in cloned cattle derived from FFB and FOV donor cells. The results demonstrated that the modification pattern of H3 K4m2 in the ear tissue of all clones was largely consistent with that of the donor cell line FFB and conventionally pro- duced cattle, but different from that of the donor cell line FOV.
作者 曹更生 张洪 周文平 李宁
机构地区 河南大学生命科学学院 中国农业大学农业生物技术国家重点实验室
出处 《遗传》 CAS CSCD 北大核心 2009年第6期611-614,共4页 Hereditas（Beijing)
基金 国家自然科学基金项目(编号:30771216)资助
关键词 免疫荧光染色 X染色体 H3 K4m2 表观遗传修饰 克隆牛 immunofluorescence staining X chromosome H3 K4m2 epigenetic modifications cloned cattle
分类号 Q343 [生物学—遗传学]
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参考文献13

  • 1Kishigami S,Wakayama S,Hosoi Y,Ieitrani A,Wakayama T.Somatic cell nuclear transfer:,infinite reproduction of a unique diploid genome.Exp Cell Res,2008,314(9):1945-1950.
  • 2Yang X,Smith SL,Tian XC,Lewin HA,Renard JP,Wakayama T.Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning.Nat Genet,2007,39(3):295-302.
  • 3Kanka J.Nuclear transplantation:reprogramming of transplanted nuclei.Reprod Nutr Dev,1999,39(5-6):545-554.
  • 4Rideout WM,Eggan K,Jaenisch R.Nuclear cloning and epigenetic reprogramming of the genome.Science,2001,293(5532):1093-1098.
  • 5Ciccodicola A,D'Esposito M,Esposito T,Gianfrancesco F,Migliaccio C,Miano MG,Matarazzo MR,Vacca M,Franzè A,Cuccurese M,Cocchia M,Curci A,Terracciano A,Torino A,Cocchia S,Mercadante G,Pannone E,Archidiacono N,Rocchi M,Schlessinger D,D'Urso M.Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region.Hum Mol Genet,2000,12; 9(3):395-401.
  • 6Disteche CM,Filippova GN,Tsuchiya KD.Escape from X inactivation.Cytogenet Genome Res,2002,99(1-4):36-43.
  • 7Bernstein BE,Kamal M,Lindblad-Toh K,Bekiranov S,Bailey DK,Huebert D J,McMahon S,Karlsson EK,Kulbokas EJ 3rd,Gingeras TR,Schreiber SL,Lander ES.Genomic maps and comparative analysis of histone modifications in human and mouse.Cell,2005,120(2):169-181.
  • 8Rougeulle C,Navarro P,Avner P.Promoter-restricted H3 Lys 4 di-methylation is an epigenetic mark for monoallelic expression.Hum Mol Genet,2003,12(24):3343-3348.
  • 9Benevolenskaya EV.Histone H3K4 demethylases are essential in development and differentiation.Biochem Cell Biol,2007,85(4):435-443.
  • 10Boggs BA,Cheung P,Heard E,Spector DL,Chinault AC,Allis CD.Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes.Nat Genet,2002,30(1):73-76.

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2009年 第6期

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