多孔性三维结构生物材料促进运动性韧带损伤重建的实验研究

Experimental Research on Application of Biomaterial with Porous and Three-Dimensional Structure During Anterior Cruciate Ligament Reconstruction

目的探讨多孔性三维立体结构生物材料—重组和异种骨(RBX)在运动性前交叉韧带断裂后进行重建过程中的应用效果。方法对15只新西兰白兔实施双侧前交叉韧带重建术,其中一侧移植物两端固定有RBX,另一侧作为对照组,分别于术后2,6和12周取材,进行MicroCT扫描以及组织学染色检测。结果结果显示术后6,12周RBX组腱骨之间组织的BMD数值均高于对照组;组织学则显示术后RBX组较对照组更早,更多的新骨形成。结论RBX可显著提高前交叉韧带重建术后腱骨之间的愈合,较早恢复膝关节功能。

Objective To investigate the effect of recombined bone xenografts （RBX） on the healing of tendon to bone through the way of imageology, histology. Methods ACL reconstruction was performed bilaterally in 15 skeletally mature rabbits using long digital extensor tendon grafts. RBX were implanted into the treated knee of each rabbit, with the contralateral knee serving as a control. After rabbits were killed at postoperative weeks 2, 6 and 12 for routine histology, the samples were then processed through Micro CT and subsequent toludine blue staining, trichrome stain. Results 6,12 weeks after operation, the values of BMD in RBX group were higher than the values in control group（ P 〈 0.05） ;Histological findings of the interface between the tendon and bone were different in the two groups. Conclusion RBX can augment the healing of tendon to bone after ACL reconstruction in a rabbit model.