中等负荷跑台训练对D-半乳糖致衰老大鼠基底前脑-皮质自由基代谢的影响

Effect of Moderate-load Platform-Running Training on Free Radical Metabolism of Basal Forebrain and Cortex

目的:观察中等负荷跑台训练对D-半乳糖致衰老大鼠基底前脑、海马结构和皮质自由基代谢的影响。方法:将30只大鼠随机分为正常对照组、衰老模型对照组和衰老模型中等负荷训练组,每组10只,6周后,用Morris水迷宫试验进行学习记忆能力的测定,然后检测基底前脑、海马结构和大脑皮质NO、丙二醛(MDA)的含量,同时测定超氧化物歧化酶(SOD)的活性。结果:衰老模型组基底前脑、海马结构和大脑皮质NO、MDA含量分别较正常对照组显著增高,而SOD活性显著降低(P<0.01);衰老模型中等负荷训练组基底前脑、海马结构和大脑皮质NO、MDA含量分别较衰老模型组明显降低;而SOD活性明显增高(P<0.05);Morris水迷宫试验结果:正常对照组潜伏期显著短于衰老模型组(P<0.01),衰老模型中等负荷训练组潜伏期显著短于衰老模型对照组(P<0.05)。结论:中等负荷跑台训练可通过提高基底前脑海马/大脑皮质系统SOD的活性,减少NO、MDA的生成,达到清除自由基,抗氧化进而延缓衰老的作用。

Tnis experiment is aimed to exam the effect of the moderate- load platform - nmning training on the free radical metabolism of the aging D - galactese and rat - model forebrain cortex by observing the thirty SD rats, which are randomly divided into three groups： nomal one, aging- model one and the aging- model moderate - load platform - running training one, and each group has 10 of them. Six weeks later, the mensuration of the spatial learning and memory ability will be tried by the Morris water maze, which will be followed by the testing of the contents of NO and malondialdehyde（MDA） in the basal forebrain, hippocampus and cereral cortex and the activities of superoxide dismute（SOD） at the same time. It is expected to show that the contents of NO and MDA in the basal forebrain, hippocampns and cerebral cortex has increased observably while the acitivities of SOD decreased evidently（ P 〈 0.01 ） compared with the nomal one. On the contrary, in the aging - model moderate - load training group, compared with the agong model one, the contents of NO and MDA in the basal forebrain, hippocampus and cerebral cortex has decreased dramatically while the acitivities of SOD increased evidently（ P 〈 0.05）. As a result, the trial of the Morris water maze shows that the latent period of the normal control group is shorter than that of the aging control group（ P 〈 0.01 ）. And the latent period of the aging moderate load training groups is shorter than that of the aging control group（ P 〈 0.05）. So we can get a conclusion that the moderate- load platform- running training can clean out the free radicals and be against aging by increasing the activities of SOD and decreasing the forming of NO and MDA.