血小板源生长因子受体α和β在瘢痕疙瘩成纤维细胞中表达的差异性研究

Different PDGFR-α and PDGFR-β expression in keloid fibroblasts

目的:研究血小板源生长因子受体(platelet-derived growth factor receptor,PDGFR)-α和PDGFR-β在瘢痕疙瘩成纤维细胞中的表达差异。方法:分别应用免疫细胞化学、western blot及荧光定量RT-PCR技术,检测瘢痕疙瘩成纤维细胞和正常皮肤成纤维细胞中PDGFR-α和PDGFR-β的蛋白和mRNA表达。结果:与正常皮肤成纤维细胞相比,瘢痕疙瘩成纤维细胞中PDGFR-α的蛋白和mRNA表达都显著升高(P=0.00,0.00,0.02),而PDGFR-β蛋白和mRNA表达的升高不显著(P=0.07,0.06,0.06)。结论:PDGFR-α表达升高与瘢痕疙瘩形成密切相关,可能是其病因机制之一。

Objective To study the different PDGFR-a and PDGFR-13 expression in keloid fibroblasts. Methods In fibroblasts derived from keloid tissue and normal skin, the protein expression and mRNA expression of PDGFR-α and PDGFR-β were detected by immunocytochemistry staining, western blot and real-time quantitative PCR respectively. Results Compared with those in normal dermal fibroblasts, The protein and mRNA expression of PDGFR-α （P= 0.00,0.00,0.02）but not PDGFR-β （P=0.07,0.06,0.06） increased significantly in keloid fibroblasts. Conclusions The elevated level of PDGFR-α is involved in keloid formation and may play certain role in its pathogenesis.