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c-Ski在糖尿病肾病大鼠肾组织中的表达及意义

The expression and significance of c-Ski in kidney of diabetic rats
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摘要 目的观察糖尿病肾病（DN）大鼠肾组织c-Ski表达，探讨其在肾小管上皮向肌成纤维细胞转分化（TEMT）中的意义。方法36只Wistar雄性大鼠随机分为正常组（n=12）、模型组（n=12）和干预组[大黄酸100mg／（kg-d），n=12]。第16周处死大鼠，观察大鼠肾脏病理改变并检测肾组织中转化生长因子-β1（TGF-β1）、α-平滑肌肌动蛋白（α-SMA）、c-Ski的表达。结果DN大鼠肾小管上皮细胞c-Ski表达与TGF-β1、α-SMA表达呈负相关；干预组大鼠肾小管上皮细胞c-Ski表达较模型组高。结论c-Ski是TEMT的一个重要保护性因子。 Objective To observe the expressions of c-Ski in renal tissue of diabetic nephropathy rats, and discuss its significance. Methods Wistar male rats were randomly assigned to 3 groups： Control group, diabetic group and treatment group. Rats were killed at the 16th week after experiment. Histopathologic changes in renal tissue were observed and immunohistochemistry was performed to investigate the expression of TGF-β1, α-SMA and c-Ski. Results The expression of c-Ski had a negative correlation with TGF-β1 ancl α-SMA. Compared to diabetic group, the expression of c-Ski was significantly increased in treatment group. Conclusion c-Ski may be a protective factor of tubular epithelial-myofibrablast transformation.
作者 张义雄 宁建平
机构地区 中南大学湘雅医院肾内科
出处 《中国医师杂志》 CAS 2009年第5期611-614,共4页 Journal of Chinese Physician
基金 湖南省科技厅项目（05FJ3058,06SK3018） 湖南省卫生厅中医药科研基金（24201）
关键词 糖尿病肾病/代谢 肾小管 基因表达 转化生长因子Β 辅肌动蛋白 Diabetic nephropathies/ME Kidney tubules Gene expression Transforming growth factor beta Actinin
分类号 R285.5 [医药卫生—中药学]
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参考文献9

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中国医师杂志
2009年 第5期

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