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c-Ski在糖尿病肾病大鼠肾组织中的表达及意义

The expression and significance of c-Ski in kidney of diabetic rats
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摘要 目的观察糖尿病肾病（DN）大鼠肾组织c-Ski表达，探讨其在肾小管上皮向肌成纤维细胞转分化（TEMT）中的意义。方法36只Wistar雄性大鼠随机分为正常组（n=12）、模型组（n=12）和干预组[大黄酸100mg／（kg-d），n=12]。第16周处死大鼠，观察大鼠肾脏病理改变并检测肾组织中转化生长因子-β1（TGF-β1）、α-平滑肌肌动蛋白（α-SMA）、c-Ski的表达。结果DN大鼠肾小管上皮细胞c-Ski表达与TGF-β1、α-SMA表达呈负相关；干预组大鼠肾小管上皮细胞c-Ski表达较模型组高。结论c-Ski是TEMT的一个重要保护性因子。 Objective To observe the expressions of c-Ski in renal tissue of diabetic nephropathy rats, and discuss its significance. Methods Wistar male rats were randomly assigned to 3 groups： Control group, diabetic group and treatment group. Rats were killed at the 16th week after experiment. Histopathologic changes in renal tissue were observed and immunohistochemistry was performed to investigate the expression of TGF-β1, α-SMA and c-Ski. Results The expression of c-Ski had a negative correlation with TGF-β1 ancl α-SMA. Compared to diabetic group, the expression of c-Ski was significantly increased in treatment group. Conclusion c-Ski may be a protective factor of tubular epithelial-myofibrablast transformation.
作者 张义雄 宁建平
机构地区 中南大学湘雅医院肾内科
出处 《中国医师杂志》 CAS 2009年第5期611-614,共4页 Journal of Chinese Physician
基金 湖南省科技厅项目（05FJ3058,06SK3018） 湖南省卫生厅中医药科研基金（24201）
关键词 糖尿病肾病/代谢 肾小管 基因表达 转化生长因子Β 辅肌动蛋白 Diabetic nephropathies/ME Kidney tubules Gene expression Transforming growth factor beta Actinin
分类号 R285.5 [医药卫生—中药学]
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参考文献9

  • 1Chen WJ,Lam SS,Srinath H,et al.Competition between Ski and CREB-binding protein for binding to Smad proteins in transforming growth factor-beta signaling.J.Biol.Chem,2007,282(15):11365-11376.
  • 2Takeji M,Kawada N,Moriyama T,et al.CCAAT/enhancer-binding protein contributes to myofibroblast transdifferentiation and renal disease progression.J Am Soc Nephrol,2004,15(9):2383-2390.
  • 3Phanish MK,Wahab NA,Colville-Nash P,et al.The differential role of Smad2 and Smad3 in the regulation of pro-fibrotic TGF beta 1 responses in human proximal-tubule epithelial cells.Biochem J,2006,393:601-607.
  • 4Picard N,Baum O,Vogetseder A,et al.Origin of renal myofibroblasts in the model of unilateral ureter obstruction in the rat.Histochem Cell Biol,2008,130(1):141-155.
  • 5刘伦志,宁建平.大黄酸对糖尿病大鼠肾小管上皮细胞表型转化的影响[J].内科急危重症杂志,2007,13(1):33-35. 被引量：7
  • 6Liu XD.Inflammatory Cytokines Augments TGF-beta 1-Induced Epithelial-Mesenehymal Transition in A549 Cells by Up-Regulating T beta R-I.Cell Motil Cytoskeleton,2008,65(12):935-944.
  • 7Fukasawa H,Yamamoto T,Togawa A,et al.Uhiquitin-dependent degradation of SnoN and Ski is increased in renal fibrosis induced by obstructive injury.Kidney Int,2006,69:1733-1740.
  • 8Le Scolan,Luo Kunxin.Ski,SnoN,and Akt as negative regulators of Smad activity:Balancing cell death and cell survival.Cancer Drug Discovery and Development,2008,139-153.
  • 9余佳,吴晓晴,孙海峰,吉民.大黄酸及其衍生物的生物活性研究进展[J].药学与临床研究,2008,16(2):125-128. 被引量：33

二级参考文献27

  • 1赵雅妮,李惊子,王海燕.血管紧张素Ⅱ受体拮抗剂与血管紧张素转换酶抑制剂抗大鼠肾脏纤维化的作用及机制探讨[J].中华老年多器官疾病杂志,2002,1(1):36-40. 被引量：24
  • 2雷作熹,罗仁,董晓蕾,钟先阳.STZ诱导糖尿病肾病大鼠模型的建立[J].中国实验动物学报,2005,13(3):163-165. 被引量：83
  • 3李灵芝,崔勇,崔颖,龚海英,张永亮.大黄酸-雌激素偶联物的合成及其药理学活性[J].中国药科大学学报,2007,38(1):6-11. 被引量：12
  • 4Iwano M,Plieth D,Danoff TM.Evidence that fibroblasts derive from epithelium during renal interstitial fibrosis.J Clin Invest,2002,110:341.
  • 5Tong SQ, Yan JZ. Large-scale separation of hydroxyanthraquinones from Rheum palmatum L. by pH-zone-refining counter-current chromatography[ J]. J Chrom, 2007, 1176( 1-2) : 163 - 168.
  • 6Gonnot V, Tisserand S, Nicolas M, et al. Total synthesis of rhein and diacerhein via a directed ortho metalation of an aromatic substrate [ J ]. Tetrahedron Lett, 2007, 48 (40) : 7117 -7119.
  • 7Tisserand S, Baati R, Nicolas M, et al. Expedient Total Syntheses of Rhein and Diacerhein via Fries Rearrangement[J]. J Org Chem, 2004, 69(25): 8982-8983.
  • 8Martel-Pelletier J, Mineau F, Jolicoeur FC, et al. In vitro effects of diacerhein and rhein on interleukin 1 and tumor necrosis factor-alpha systems in human osteoarthritic synovium and chondrocytes[J]. J Rheumatol,1998, 25(4) :753 -762.
  • 9Lemay S, Mao CC, Singh AK. Cytokine gene expression in the MRL/lpr model of lupus nephritis[J]. Kidney Int, 1996, 50 (1) :85 -93.
  • 10Tamura T, Kosaka N, Ishiwa J, et al. Rhein, an active metabolite of diacerein, down-regulates the production of pro-matrix metalloproteinases-1 ,-3,-9 and-13 and up-regulates the production of tissue inhibitor of metalloproteinase-1 in cultured rabbit articular chondrocytes[ J ]. Osteoarthr Cartilage, 2001, 9 (3) : 257 - 263.

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中国医师杂志
2009年 第5期

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