纳米金共振光散射猝灭法测定磷

Resonance light scattering quenching determination of phosphorus with nanogold

在0．013mol／L H2SO4介质中，磷酸根与钼酸钠、酒石酸锑钾反应生成淡黄色的磷锑钼杂多酸，加入纳米金后在710nm处产生一个较强的共振散射峰，抗坏血酸可将磷锑钼杂多酸还原为磷锑钼蓝，导致710nm处共振光散射信号降低。磷浓度在0．033-49．95ng／mL范围内与共振光散射强度降低值呈良好线性关系，磷检出限为0．01ng／mL。拟定的方法用于水样中磷的测定，测定结果与分光光度法的测定结果一致。

In the medium of 0. 013 mol/L H2 SO4, phosphate reacts with sodium molybdate and potassium antimonyl tartrate, forming a yellowish phosphorus-antimony-molybdenum heteropoly acid. The acid exhibited a strong resonance scattering peak at 710 nm after added with nanogold. The heteropoly acid was reduced by ascorbic acid to antimony phosphomolybdate blue, which caused the decrease of resonance scattering intensity. Phosphorus concentration in the range of 0. 033--49.95 ng/mL is linear to the decrease of resonance scattering intensity, with detection limit of 0.01 ng/mL. The present method has been applied in the determination of phosphate in water, and the results were agreement with those of spetrophotometry.