4个芦荟品种离体快繁技术研究

Studies on in vitro rapid propagation of 4 Aloe species

该文对库拉索、中华、木立和高尚4个芦荟品种离体快繁技术进行了研究。结果表明,库拉索和木立芦荟不定芽间接分化,库拉索主要是丛生芽+愈伤组织方式,木立芦荟主要是愈伤组织的形式;中华芦荟和高尚芦荟主要是丛生芽方式直接分化。4个芦荟品种的最适继代增殖培养基库拉索为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,繁殖系数为7.29;中华芦荟为MS+6-BA 1.0 mg/L+NAA 1.5 mg/L,繁殖系数达6.64;木立芦荟为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L,繁殖系数达14.0;高尚芦荟为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,繁殖系数达8.10。库拉索和中华芦荟的最适生根培养基为MS+NAA 0.5 mg/L,生根率分别为86.5%和100%;木立和高尚芦荟为MS,生根率分别为99%和79%。最适移栽基质为珍珠岩∶河沙∶草炭土=1∶1∶1,成活率达92%。

In this paper, studies on in vitro propagation of 4 Aloe species were conducted. The results showed that： the main ways of dedifferentiation forAloe vera, Aloe vera vat. chinensis, Aloe arborescens and Aloe nobilis were adventitious shoots and callus, adventitious shoots, callus and adventitious shoots respectively. Regarding the optimal subculture media, Aloe barbadensis was Ms ＋ 6BA2.0＋NAA0.1, the breeding coefficient was 7.29; Aloe vera vat. chinernsis was Ms＋6BA1.0＋NAA1.5, the breeding coefficient was 6.64; Aloe vera was Ms＋6BA2.0＋NAA0.1, the breeding coefficient wasl4.0; Aloe nobilis was Ms＋6BA1.0＋NAA0.1, the breeding coefficient was 8.10. The optimal roofing culture media were MS or MS＋NAA0.5, the roofing rates were 86.5%, 1130%, 99% and 79% respectively. The optimal transplant media were mixture of pearl rock, sand and peat soil with the 1 ： 1 ： 1 ratio, and the survival rates might attain 92%.