p^(53)基因与Rb基因在卵巢癌组织中突变的初步分析

Preliminary analysis of p53 gene and Rb gene mutation in ovarian cancer tissue.

为了解卵巢癌组织中抗癌基因ｐ５３与Ｒｂ基因的突变情况，我们把ＰＣＲ单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术及ＰＣＲ－ＤＮＡ直接测序方法应用于５７例卵巢癌组织细胞的检测。结果发现ｐ５３基因可能的突变率为３２％，Ｒｂ基因可能的突变率为２１％，不同分化程度肿瘤的ｐ５３及Ｒｂ基因突变率未见明显区别。结论，ＰＣＲ－ＳＳＣＰ银染技术是筛查基因突变简便、敏感、无核素污染的最佳方法。

Objective: To recognize the mutation status of p53 and Rb cancer suppresor genes. Methods: for the first time, 57 ovarian cancer samples were analysised by PCR for DNA amplification, and then SSCP, furthes PCR DNA sequence. Results:(1) The mutation ratio of p53 in ovarian cancer was 32%, (2) The mutation of Rb gene in ovarian cancer was 21%, (3)Neither p53 nor Rb gene mutation ratio was found significant difference in varions differential stages. Conclusions: PCR SSCP DNA sequence method is the best one in testing gene mutation for its simplicity, sensitivity and no radioisotope contamination. Direct PCR DNA sequence can test point mutation of single amino acid change.