蝎毒多肽提取物抑制胰腺癌肝转移作用的实验研究

Polypeptide extract from scorpion venom inhibits liver metastasis in pancreatic cancer

目的探讨东亚钳蝎蝎毒多肽提取物(PESV)对胰腺癌肝转移的抑制作用。方法筛选出高肝转移的胰腺癌细胞亚型MIA-PaCa2-3,用Transwell小室模型观察PESV对MIA-PaCa2-3细胞体外侵袭能力的影响;尾静脉注射MIA-PaCa2-3细胞建立高肝转移率的裸鼠胰腺癌模型,对比PESV和化疗药物吉西他滨(gemcitabine)以及抗血管生成剂TNP-470对裸鼠胰腺癌肝转移的抑制作用。结果PESV可以抑制MIA-PaCa2-3细胞穿过人工基底膜的能力;PESV处理组肝转移抑制率为70%(7/10),与吉西他滨处理组相近(8/10),明显高于TNP-470处理组(2/9)。结论PESV作为一种"抗血管-细胞毒"药物对胰腺癌肝转移具有明显的抑制作用。

Objective To explore the inhibitory effect of polypeptide extract from scorpion venom （PESV） on liver metastasis in pancreatic cancer. Methods A sub-hne of human pancreatic cancer cell line MIA-PaCa2 （MIA-PaCa2-3） with high liver metastasis potential was selected and injected through the tail vein to establish a pancreatic cancer model in nude mice with high liver metastasis. The capability of invasion in vitro was evaluated by a transwell chamber system. The inhibitory effects of PESV, chemotherapoutic agent gemcitabine, and angiogenesis inhibitor TNP-470 on liver metastasis in the pancreatic cancer model were compared. Results The capability of invasion of the MIA-PaCa2-3 cells was significantly decreased by PESV, and liver metastasis was significantly inhibited in nude mice models （7/10） by PESV, which was similar to that in the gemcitabine-treated group （8/10） and higher than that in the TNP-470-treated group. Conclusion PESV, as a novel angiocytotoxie agent, has an inhibitory effect on liver metastasis in pancreatic cancer.