慢性ITP小鼠外周血IL-11及其受体水平的研究

IL-11 and IL-11 receptor levels in the blood of mice with chronic idiopathic thrombocytopenic purpura

目的测定慢性特发性血小板减少性紫癜(CITP)小鼠外周血中IL-11及其受体(IL-11R)的水平,探讨CITP的发病机制,为ITP的治疗提供新的理论依据。方法BALA/C小鼠60只,随机分为对照组(20只)和CITP组(40只),通过腹腔注射豚鼠抗小鼠血小板血清(APS)的方法建立CITP模型。于造模第8d分别采用ELISA、流式细胞术测定两组小鼠外周血中IL-11及IL-11R的水平。结果CITP小鼠外周血中IL-11及IL-11R平均水平均较对照组升高(P<0.05);其中有31只小鼠同时存在IL-11及IL-11R水平的升高,余9只小鼠仅存在IL-11水平的升高,而IL-11R水平降低。结论IL-11及IL-11R在CITP的发病机制中起一定作用,但它们发挥作用的原理及过程尚需进一步探讨。

Objective To explore IL- 11 and IL- 11 receptor in the blood of mice with chronic idiopathic thrombocytopenic purpura （CITP） and to assess whether the absence of IL-11 or the IL-11 receptor will provide a new theory in the treatment of CITP. Methods 60 BALB/C mice which were randomly divided into two groups （20 mice in the control group and 40 in the C/TP group）. The model of CITP was induced using intra-peritoneal APS injection. Levels of IL-11 and IL-11 receptors were deter- mined using ELISA and flowcytometry at the 8th day. Results The levels of IL-11 and the IL-11 receptor in the blood of group CITP were higher thatthose of group control （ P 〈0.05）. In 31 mice of the CITP group,both the level of IL-11 and of the IL-11 receptor in the blood were increased, while in the remaining 9 mice of the CITP group,the level of IL-11 in the blood was increased but the level of the IL- 11 receptor was decreased. Conclusion IL-11 may have a certain action in the mechanism of ITP.