PDCD4和Ki67蛋白在不同期蕈样肉芽肿中的表达

Expressions of programmed cell death 4 and Ki67 in granuloma fungoides

目的探讨程序性细胞凋亡因子4(PDCD4)和Ki67蛋白在不同期蕈样肉芽肿(MF)皮损中的表达。方法选择27例MF病变皮肤及5例正常皮肤组织蜡块,分为:红斑/斑块期MF(n=21)、肿瘤期MF(n=6)和正常对照组(n=5),应用免疫组织化学Envison两步法分别检测3组标本表皮及真皮中PDCD4及Ki67的表达。结果①红斑/斑块期MF皮损表皮PDCD4+细胞与正常对照组比较差异无统计学意义(P>0.05),真皮PDCD4+细胞明显少于正常对照组(P<0.05);肿瘤期MF皮损表皮及真皮PDCD4+细胞均少于正常对照组和红斑/斑块期MF(P<0.05);②两组MF表皮Ki67+细胞的数量与正常对照组比较差异无统计学意义(P>0.05),红斑/斑块期MF及肿瘤期MF皮损真皮Ki67+细胞均多于正常对照组(P<0.05);肿瘤期MF皮损真皮Ki67+细胞高于红斑/斑块期MF皮损(P<0.05);③各期MF的表皮及真皮中PDCD4和Ki67的表达无相关性(P>0.05)。结论PDCD4的表达从MF的红斑/斑块期到肿瘤期逐渐减低,真皮中Ki67的表达逐渐增强,MF的发生与发展可能与PDCD4表达降低或缺失有关。

Objective To explore the expressions of Programmed Cell Dead Factor4 and Ki67 in the skin infiltrates of patients with granuloma fungoides（MF） in different stages. Methods Paten sections were divided into three groups： the patch/plaque stage group（ n = 21）, the tumor stage group（ n = 6）, and the control group（ n = 5）. The immunohistochemical Envision method was used to determine expressions of PDCIM and Ki67 per section. Results OThere was no significant difference in the number of PDCD4^＋ cells in the epidermis between the patch/plaque stage and control groups （ P 〉 0.05）, while the number of PDCD4^＋ cells in the derails was significantly lower than that in the control group （ P 〈 0.05）. The number of PDCD4^＋ cells in both the epidermis and dermis of the tumor stage group was significantly lower than in the patch/plaque stage and control groups （P〈0.05）.② There was no significant difference in the number of Ki67＋ cells in the epidermis in the three groups （P 〉 0.05）. The number of Ki67 ＋ cells in dermis of beth the patch/plaque stage and the tumor stage groups were significantly higher than the control group （ P 〈 0.05）, while the number of Ki67^＋ cells of the tumor stage group was significantly higher than that of the patch/plaque stage group （ P 〈0.05）. （3）There was no correlation between expression of PDCD4 and that of Ki67 in the epidermis or dermis （P 〉 0.05）. Conclusion Decreased PDCD4 expression in both the epidermis and dennis and in- creased Ki67 expression in the dennis from patch/plaque stage to tumor stage MF suggest that the incidence and development of MF may be related to the decrease or deficiency of PDCD4 expression.