子痫前期患者白细胞介素-8和胎盘生长因子的表达及其意义

Expression and Significance of Interleukin-8 and Placenta Growth Factor in Patients with Preeclampsia

目的通过检测子痫前期患者羊水中白细胞介素-8(IL-8)和胎盘组织中胎盘生长因子(PLGF)的水平,以探讨IL-8和PLGF在子痫前期发病中的作用。方法采用酶联免疫吸附法和免疫组化法分别测定20例轻度子痫前期患者,22例重度子痫前期患者和30例正常妊娠妇女(对照组)的羊水中IL-8和胎盘组织中的PLGF的表达。结果子痫前期轻度组及重度组羊水IL-8均高于对照组,重度组羊水IL-8显著高于轻度组,差异均有统计学意义(P<0.01)。子痫前期轻度组及重度组胎盘组织中PLGF表达量均低于对照组(P<0.01),而重度组低于轻度组(P<0.01)。子痫前期轻、重度组及对照组胎盘组织中PLGF表达量与羊水IL-8水平均呈高度负相关(P<0.01)。结论子痫前期患者羊水中IL-8增高,而胎盘组织中PLGF表达下降,可能与子痫前期的发生、发展有关。

Objective To investigate the expression levels of interlenkin-8 （IL-8） in the amniotic fluid and placenta growth factor （PLGF） in placenta and their correlation with preeclampsia. Methods Amniotic fluid and placenta samples were collected from normal pregnant woman 30 cases（ control group）, mild preeclampsia group 15 cases and severe preeclampsia group 20 cases. IL-8 level were determined with enzyme-linked immunosorbent assay （ELISA）, and PLGF expression were determined with immunohistochemistry. Results The levels of IL-8 in the mild preeclampsia and severe preeclampsia groups were significantly higher than that of the control group （P〈0.01）, and the level of severe preeclampsia group was significantly higher than that of mild preeclampsia group （P〈0.01）. The PLGF expression levels of mild preeclampsia and severe preeelampsia groups were significantly lower than that of control group （P〈0.01）, and the level of severe preeclampsia group was significantly lower than that of mild preeclampsia group（P〈0.01 ）. IL-8 level is negatively correlated with PLGF expression （P〈0.01）. Conclusion The increase in the amniotic fluid level of IL-8 and the decrease in the placental level of PLGF may be correlated with the occurrence and development of preeclampsia.