重组人睫状神经营养因子对胶质瘤细胞粘附性和细胞膜流动性的影响

EFFECTS OF RECOMBINANT HUMAN CILIARY NEUROTROPHIC FACTOR ON THE CELL ADHESIVENESS AND MEMBRANE FLUIDITY OF GLIOMA CELL LINE

观察了重组人睫状神经营养因子（ｒｈＣＮＴＦ）对大鼠胶质瘤Ｃ６细胞粘附性和细胞膜流动性的影响。发现２００ｎｇ／ｍｌ～２０００ｎｇ／ｍｌｒｈＣＮＴＦ可使Ｃ６细胞在层粘蛋白基质上的粘附性显著降低，细胞膜流动性增加。结果表明，ｒｈＣＮＴＦ对Ｃ６细胞的生长抑制作用可能与其改变瘤细胞膜及表面结构物质有关。

This study was designed to observe the effects of recombinant human ciliary neurotrophic factor(rhCNTF)on the cell adhesiveness and cell membrane fluidity of rat glioma C 6 cell line. The results showed that the adhesiveness of C 6 cells to laminin substrate as obviously inhibited by rhCNTF 200 ng/ml～2000 ng/ml. The cell membrane fluidity increased markedly after rhCNTF treatment. We think that the effects of rhCNTF on the proliferation of rat glioma C 6 cell might be related to the changes of the outward structure of the cell membrane, and it may play a potential role as a cellular regulator in the treatment of glioma.