侧脑室注射IL-1ra后脑缺血大鼠下丘脑室旁核CRH的表达

The Expression of Corticotropin-releasing Hormone in the Hypothalamic Paraventricular Nuclei of Cerebral Ischemic Rat After Microinjection of Interleukin-1 Antagonist into Lateral Ventricule

目的探讨白介素－１（ＩＬ－１）是否参与脑缺血大鼠下丘脑－垂体－肾上腺轴（ＨＰＡ）的激活。方法选用成年ＳＤ大鼠，分为１７组（Ａ～Ｑ组），Ａ组为正常对照组，Ｂ～Ｏ组大鼠脑缺血时间分别为１、３、６、２４、４８、７２ｈ及１、２、３、４、５、６、７、８周；Ｐ组的处理为：用微量注射泵将ＩＬ－１受体拮抗剂（ＩＬ－１ｒａ）注入大鼠侧脑室，之后建立脑缺血模型；Ｑ组为Ｐ组的对照组，用微量注射泵将生理盐水注入大鼠侧脑室，之后建立脑缺血模型。用免疫组化法显示各组大鼠下丘脑室旁核（ＰＶＮ）促肾上腺皮质激素释放激素（ＣＲＨ）的表达。结果正常大鼠ＰＶＮ有少量ＣＲＨ表达，脑缺血４８ｈ内ＰＶＮ无ＣＲＨ表达。从脑缺血７２ｈ起，ＰＶＮ之ＣＲＨ的表达开始增多，并且持续至脑缺血第８周。Ｐ组大鼠ＰＶＮ有较多ＣＲＨ表达，而Ｑ组大鼠ＰＶＮ无ＣＲＨ表达。结论ＩＬ－１可能通过促进下丘脑ＰＶＮ释放ＣＲＨ而参与脑缺血时下丘脑－垂体－肾上腺轴的激活。

Objective To study whether interleukin-1 ( IL- 1 ) was involved in the activation of hypothalamo-pituitary-adrenal axis (HPA ) during cerebral ischemia. Method Different ischemic time. Interleukin-1 receptor antagonist ( IL- 1ra ) was microinjected into the lateral ventricles of cerebral ischemic models with then the expression of corticotropin-releasing hormone (CRH ) in the hypothalamic paraventricular nuclei (PVN ) were observed. Results There were few CRH expression in the PVN of normal controls and no CRH expression within 24 h of cerebral ischemia, and quite a few CRH expression in PVN of group P and none in that of group Q. Conclusion IL- 1 may be involved in the activation of HPA by increasing the release of CRH.