纳米银溶胶的研制

Preparation and Evaluation of Nano-Silver Sol

目的:采用一条绿色、环保的途径合成纳米银溶胶。方法:以水溶性壳聚糖为胶体保护剂、还原剂及络合剂,葡萄糖为促进剂,通过超声振荡的方法还原硝酸银合成了纳米银溶胶。对纳米银溶胶进行了透射电镜TEM的表征和紫外分光光度的扫描,通过调整水溶性壳聚糖、葡萄糖的比例、超声波的功率、时间,以透射电镜测试结果为指标,确定了纳米银溶胶的最佳合成工艺。对纳米银溶胶的贮存稳定性进行了考察,进行了纳米银溶胶的体外抗菌性能的研究。结果:当m壳聚糖:m葡萄糖:mAgNO3=10:5:1,超声功率150W,超声60min时,所制备的纳米银溶胶的平均粒径在10nm以下,且分布比较均匀,形状为类球形;溶胶放置一年不聚集,较稳定。该纳米银溶胶体外抗菌性能测试结果表明对金黄色葡萄球菌、大肠杆菌、白色念珠菌有很明显的抑菌圈,100mg.L-1的纳米银溶胶的抑菌率都为100%。结论:该纳米银溶胶制备工艺切实可行,稳定可靠,体外抗菌试验为制剂提供了理论基础。

Objective： To study the nano-silver sol using green way. Methods： Taken the water-soluble chitin as the colloid protecting agent, the reducer and the complex agent, and the glucose as the promoter the AgNO3 was reduced to nano-silver sol through the supersonic vibration. The way of the synthesized nano-silver sol was green. The nano-silver sol was examined by TEM and the ultraviolet scanning. Through the adjustment of the ratio of the chitin and glucose to the AgNO3 and the ultrasonic wave power and time, the optimal factors of the nano-silver sol were determined by the tested results of the TEM. The nano-silver sol＇s stability had been carried on, and external antibacterial performance had been studied. Results： When the ratio of chitin, glucose, to AgNO3 was 10：5：1, the ultrasonic wave power and time was150W and 60min, the particle size of obtained nano-silver sol was 10nm below and the distribution of the particles was narrower and the shape was similar as spherical. The sol has not been congregated for 1 year. The sol had obvious antibacterial-circle to the S.aureus, the E.coli and Candida albicans, and The sterilization rate of the 100 mg.L^-1 sol was 100%. Conclusion： The optimal technology of sol was stable and feasible. The study on the external antibacterial property of the sol offered a foundation to the praeparatum.