十全大补汤对荷膀胱癌小鼠大剂量化疗的减毒增效作用

Toxicity Attenuation and Efficicacy Potentiation Effects of Shiquan Dabu Tang on High Dose of Chemotherapy in Tumor-bearing Mice

目的 探讨中药十全大补汤（Shiquan Dabu Tang，SDT）对小鼠膀胱癌大剂量化疗的减毒增效作用。方法 将膀胱癌组织块接种于T739小鼠皮下，随机分为对照组、环磷酰胺（CTX）100、200、400mg＆g组、SDT低剂量、高剂量组和SDT高剂量加CTX200mg／kg联合用药组，观察小鼠体重、肿瘤结节直径及外周血象的变化。结果 不同剂量的SDT均可有效抑制小鼠肿瘤的生长。SDT与CTX联合用药组小鼠的平均荷瘤生存时间为（49．4±2313）d，较对照组、高剂量SDT组（11．75±2．06）d、（17.4±5．77）d明显延长（P〈0．01），与大剂量CTX组（23±14．02）d比较差异有统计学意义（P〈0．05）。SDT连续用药1周后，高剂量SDT组、大剂量CTX组小鼠外周血PLT计数明显增加（P〈0．05）；SDT连续用药2周后，外周血RBC计数、HB浓度明显增高护〈0．05）。结论 高剂量SDT联合大剂量CTX化疗可提高疗效，延长荷瘤小鼠的平均生存时间，增加外周血小板数量，降低大剂量CTX对外周血红细胞的毒性作用，具有增效减毒作用。

Objective To investigate the toxicity attenuation and efficacy potentiation effects of Shiquan Dabu Tang （SDT） on high dose chemotherapy in T739 mice with bladder carcinoma. Methods Mouse bladder carcinoma tissue was inoculated subcutaneously into T739 mice to establish tumor-bearing mice model. The tumor-bearing mice were randomly divided into a CTX group （100, 200, 400 mg/Kg respectively）, a SDT group （high or low dose respectively）, a high-dose SDT combined with 200 mg/Kg CTX group and a control group, The body weight, diameter of tumor nodules and complete blood count were observed subsequently. Results Different doses of SDT could effectively inhibit tumor growth in mice. SDT ＋ CTX treatment significantly prolonged the survival time of mice by 49.4±23.3 days （P〈0.01, 0.05, 0.01）, compared with high dose SDT treatment （17.4± 5.77） days, 200 mg/kg CTX treatment （23 ± 14.02） days and control group （11.75±2.06） days respectively. The peripheral platelet count increased more significantly in mice treated with SDT within a week as compared to mice without SDT treatment （P〈0.05）. The peripheral RBC count and HB concentration increased more significantly in mice treated with SDT for 2 weeks as compared to mice without SDT treatment （P〈0.05）. Conclusions SDT could enhance the anti-tumor effects of high dose CTX on tumor-bearing mice and reduce toxicity in its peripheral red blood cells. The results showed that SDT combined with high dose of CTX chemotherapy had toxicity attenuation and efficacy potentiation effects in tumor-bearing T739 mice.