宫颈癌新辅助化疗前后细胞增殖和凋亡的研究

A study on proliferation and apoptosis of cervical carcinoma cells before and after neoadjuvant chemotherapy

目的探讨宫颈鳞癌细胞增殖和凋亡与新辅助化疗疗效的关系。方法选取30例宫颈鳞癌患者新辅助化疗前后的宫颈组织，23例宫颈上皮内瘤变组织，11例正常宫颈组织，分别应用免疫组化SP法和脱氧核糖核苷酸末端转移酶介导dUTP缺口末端标记法检测组织中的Ki-67核抗原指数和凋亡指数。结果化疗临床有效率为80％；化疗前后宫颈鳞癌组织Ki-67增殖指数表达分别为65．43±15．42和31．67±7．42，差异有统计学意义（t=12．501，P〈0．05）；凋亡指数分别为7．13±1．27和17．88±4．39，经比较差异有统计学意义（F=127．659，P〈0．05）；化疗前后有效组和无效组宫颈鳞癌组织凋亡指数变化值分别为12．21±4．38和4．95±2．69，经比较差异有统计学意义（t=3．849，P〈0．05）。结论新辅助化疗能抑制宫颈鳞癌细胞增殖，诱导凋亡；化疗后宫颈鳞癌细胞凋亡指数明显上调者可能与良好预后有关。

Objective To study relationship between therapeutic effect of neoadjuvant chemotherapy （ NACT ） and proliferation and apoptosis of cervical squamous carcinoma cells. Methods The cervical tissues of 30 women with cervical squamous carcinoma before and after neoadjuvant chemotherapy, 23 women with cervical intraepithelial neoplasia （CIN） and 11 normal women were taken and examined by SP immunohistochemical method and TdT-mediated dUTP-biotin nick end labeling（TUNEL） for Ki-67 antigen and apoptosis index（AI） in them. Results The clinically effective rate of NACT was 80% （24/30）. Before and after NACT, the expression of Ki-67 were 65.43 ± 15.42 and 31.67 ±7.42 respectively and Al were 7.13 ± 1.27 and 17.88 ±4.39 respectively, and there were both significant differences （t = 12. 501, P 〈 0.05; F = 127. 659, P 〈 0.05 ）. The Al variation value before and after NACT in the cervical tissues of patients with cervical carcinoma was 12.21 ±4.38 and 4.95 ＋ 2.69 respectively, there was significant difference between NACT non-effective group and NACT effective group（ t = 3. 849, P 〈 0.05）. Conclusion NACT could inhibit proliferation and induce apoptosis of tumor cells in cervical squamous carcinoma tissues. The significant up-regulation of AI after NACT may be related to a good prognosis of the disease.