3种酚类化合物对多刺裸腹溞GST和AChE活力的影响

Effects of Phenolic Compounds on Glutathione-S-Transferase and Acetylcholinesterase in Moina macrocopa

为探讨酚类化合物对水生生物的毒性效应,实验研究了多刺裸腹溞(Moina macrocopa)暴露于不同浓度的苯酚(0.25、0.75、1.25、1.75、2.25mg·L-1)、邻苯二酚(0.10、0.25、0.40、0.55、0.70mg·L-1)和间苯二酚(0.015、0.030、0.045、0.060、0.075mg·L-1)后,体内谷胱甘肽-S-转移酶(GST)和乙酰胆碱酯酶(AChE)活力的动态变化.结果表明,3种酚类化合物处理24h后,多刺裸腹溞体内GST活力随染毒浓度的升高呈先升高后降低趋势.处理48h后,体内GST活力随苯酚和邻苯二酚浓度的升高逐渐降低,而间苯二酚对GST活力的影响与处理24h后GST活力的变化趋势相似.3种酚类化合物处理24h、48h后,多刺裸腹溞体内AChE活力均呈先升高后降低趋势.48h处理多刺裸腹溞体内GST和AChE活力均显著低于24h处理.短时间内低浓度酚类化合物对GST和AChE活力具有诱导作用,但随着酚类化合物处理浓度的升高和处理时间的延长酶活力受到显著抑制.GST和AChE活力的变化能够灵敏地反映出酚类化合物对多刺裸腹溞的毒性大小及造成的损伤,初步推断可作为生物标志物来评价酚类污染物对水生生物的生化毒性.

To explore the toxic effects of phenolic compounds on aquatic organisms, the dynamic changes of glutathione-S7 transferase （GST）and acetylcholinesterase （AChE）activities in Moina macrocopa exposed to different concentrations of phenol （0.25, 0.75, 1.25, 1.75, 2.25mg·L^-1）, pyrocatechol（ 0.10, 0.25, 0.40, 0.55, 0.70mg·L^-1）and resorcinol（0.015, 0.030, 0.045, 0.060, 0.075mg ·L^-1）were studied. Results showed that lower concentrations of the three phenolic compounds induced the activities of GST but inhibited them at higher concentrations after 24h exposure. After 48h exposure, The GST activities were all inhibited under the exposure of phenol and pyrocatechol, but the GST activities were induced at first and then inhibited under the exposure of resorcinol. The activities of AChE were all induced at first and then inhibited under the exposure of the three phenolic compounds. The GST and AChE activities under 48h exposure were significantly lower than those of 24h exposure. Lower concentrations of the three phenolic compounds induced the activities of GST and AChE but inhibited at higher concentrations and longer time. In conclusion, the activities of GST and AChE could obviously reflect the toxicity of the three phenolic compounds to M. macrocopa. According to the preliminary analysis, GST and AChE could be served as biomarkers to assess the biochemical toxicity of the phenolic contamination to aquatic organisms.