面神经损伤后糖皮质激素受体α在面神经核及外周淋巴组织的表达

Expression of glucocorticoid receptor alpha in facial nucleus and peripheral lymphoid tissues after facial nerve injury

目的探讨面神经损伤后,糖皮质激素受体α(GRα)在面神经核团及外周淋巴组织的表达和变化规律。方法建立新西兰兔面神经损伤模型,分别于损伤后24 h4、8 h7、2 h、7 d1、4 d取出面神经核团和颈部淋巴结,采用免疫组化SP法,观察糖皮质激素受体的表达情况。结果糖皮质激素受体α在面神经核团及外周淋巴细胞中均有表达;糖皮质激素受体α表达增高在面神经核中出现在损伤48 h后,颈部淋巴结出现在损伤72 h后。结论糖皮质激素受体α表达在面神经核团及外周淋巴细胞胞核及胞质中,在面神经受到损伤后其表达量增高,而且神经损伤较单纯外伤可以引起较长时间的局部炎症反应。

Objective To determine expression of glucocorticoid receptor alpha （GRa） in facial nucleus and peripheral lymphoid tissues. Methods A rabbit model of facial nerve injury was estabhshed and facial nucleus and cervical lymph nodes were taken out after 24 h, 48 h, 72 h, 7 d, and 14 d to observe expression of glucocorticoid receptor alpha using immunohistochemistry. Results Expression of glucecorticoid receptor alpha was detected in facial nucleus and peripheral lymph cells and began to increase at 48 h in the facial nucleus at 72 h in cervical lymph nodes. Conclusions Expression of glucocorticoid receptor alpha was detected in the nuclei and cytoplasm in facial nucleus and peripheral lymph cells and was increased after facial nerve injuries. Local inflammatory reaction triggered by nerve injuries lasted for a longer duration than that caused by mere traumas.