还氧合酶-2与鼻息肉发病的关系

COX-2 and nasal polyps

目的探讨还氧合酶-2(COX-2)在鼻息肉组织中的表达,分析它在鼻息肉发病过程中的作用,为鼻息肉发病机制的研究提供理论依据,并为鼻息肉的药物治疗寻找新的特异性靶点。方法选取34例未应用任何药物的鼻息肉手术组织标本,根据1997年海口会议标准,分为鼻息肉A组(Ⅱ型12、期鼻窦炎鼻息肉)和鼻息肉B组(Ⅱ型3期及Ⅲ型鼻窦炎鼻息肉)。同时取10例手术患者的下鼻甲游离缘作为正常对照。采用免疫组织化学SP法对鼻息肉和正常下鼻甲黏膜组织中的COX-2进行观测并拍照。采用JD901图像分析软件分析阳性染色面积和染色密度。结果COX-2在正常下鼻甲黏膜组织中无或极少量表达,在鼻息肉组织中均显示高表达(P<0.01);Ⅲ型鼻息肉组织中的阳性表达面积和密度均高于Ⅱ型鼻息肉组(P<0.05)。结论COX-2在鼻息肉组织中高表达,且与鼻息肉的分型有关,表明其催化产物PGE2参与了鼻息肉的发生发展。

Objective To explore the effect of COX-2 on nasal polyps to search for a new special drug target for nasal polyps. Methods 34 cases of nasal polyps did not use any drugs. Based on the Haikou standard （1997）, they were divided two groups： the nasal polyp A group（type Ⅱ stageland 2）and the nasal polyp B group（type Ⅱ stage3 and type Ⅲ nasosinusitis nasal polyps）. 10 samples taken from free margins of the concha nasalis inferior served as the controls. By an immunohistochemical SP method, COX-2 protein was detected in nasal polyps and normal concha nasalis inferiors. Using the JD901 image analysis software, we de- termined the positive staining of cells and dyeing density. The results were analyzed by T test and hnear correlation using SPSS statistic software. Results Protein expression of COX-2 was negative or lowly expressed in the normal mucosa. All sections of nasal polyps showed a significantly high expression of COX-2 （ P 〈 0.01 ）. Positive area and density of COX-2 in type Ⅲ were higher than those in type Ⅱ cases（ P 〈 0.05）. Conclusion COX-2 is over expressed in nasal polyps. Its expression level is different in the two types of nasal polyps. This result indicates that the PGE2 pathway participates in the genesis and development of nasal polyps.