水稻成熟胚愈伤组织对细条病菌的抗性筛选

Screening on resistance to pathogen of bacterial leaf streak among callus derived from mature embryo of rice

摘要：以感病水稻品种汕优63、V优64为材料，探讨不同激素对水稻成熟胚愈伤组织诱导与分化的影响，并对细菌性条斑病病原菌滤液毒素对愈伤组织增殖与生长以及分化苗的影响进行研究。结果表明，以改良N6为基本培养基，添加1．5mg／L2，4-D对愈伤组织诱导和增殖有明显的促进作用，0．5mg／L6-BA与2，4-D组合可提高愈伤组织诱导率和增殖率。在愈伤组织增殖分化培养过程中，随着病原菌滤液浓度的增加，愈伤组织的增殖率不断下降、褐变严重。在继代分化培养中，随着培养基中细条病病原菌滤液浓度的增加，接种的抗性愈伤组织分化苗数、抗性苗比例不断减少，在40-55mL/L的选择压力下，分别得到中抗植株25、14株。因此，利于水稻成熟胚愈伤组织分化并获得抗性苗的细条病病原菌菌液浓度为40-55mL／L。

In the experiment, susceptible rice varieties Shanyou 63 and V You 64 were used as materials to study the effects of different hormones on the callus induction and differentiation of mature embryo of rice, and the effects of different pathogen concentrations of bacterial leaf streak on the multiplication and growth of callus and the differentiated plantlets. The results showed that when the improved N6 was used as basal medium adding 1.5 mg/L 2,4-D in medium had obvious effects on improving the induction and multiplication of callus, and adding 0.5 mg/L 6-BA and 1.5 mg/L 2,4-D could increase the induction rate and multiplication rate of callus. Dating the multiplication and differentiation culture of callus, the multiplication rate of callus was decreased and the callus had severe browning with the increase of pathogen concentration. During the subculture of callus, with the increase of pathogen concentration, the differentiation amount of resistant callus and resistant plantlet rate were decreased, and the moderate resistant plant number in field was 25 and 14 when 40 mL/L and 55 mL/L pathogen concentrations were added to the medium, respectively. Therefore, the suitable pathogen concentration of bacterial leaf streak for the callus differentiation of mature embryo of rice and obtaining resistant plantlet was 40-55 mL/L.