紫苏不同外植体组培灭菌条件研究

Sterilization conditions for tissue culture of different explants derived from Perilla frutescens (Linn) Britt

分别以紫苏种子、子叶、下胚轴、叶片为外植体,采用不同酒精、升汞、NaClO灭菌时间进行处理,探讨不同处理对外植体污染和生长的影响以及不同外植体的最适灭菌条件。结果表明,不同酒精、升汞、NaClO灭菌时间对不同外植体的影响较明显,综合发芽率、污染率或愈伤组织的诱导和生长情况,紫苏种子较好的灭菌方法为:75%酒精20s+0.1%升汞10min,污染率和发芽率分别为50%、36%;子叶、下胚轴较好的灭菌方法为:75%酒精20s+0.1%升汞4min,其污染率和愈伤组织诱导率分别为:7%、8%和40%、36%;适于叶片灭菌的条件为:75%酒精30s+0.1%升汞7min,其污染率和愈伤组织诱导率分别为10%、7%。该结果为紫苏再生体系的建立和快速繁殖技术研究奠定基础。

Seeds, cotyledons, hypocotyls and leaves of Perilla frutescens （Linn） Britt were used as explants and sterilized with different times of alcohol, mercuric chloride and NaClO to discuss their effects on the pollution and growth of explants and obtain the optimal sterilization conditions. The results showed that the different sterilized times of alcohol, mercuric chloride and NaClO had obvious effects on different explants. According to the germination rate and pollution rate, and the induction and growth of callus, the best sterilization method for seed was 75% alcohol in 20 sec and 0.1% mercuric chloride in 10 rain, which germination rate and pollution rate were 50% and 36%, respectively. The best sterilization method for cotyledons and hypocotyls was 75% alcohol in 20 see and 0.1% mercuric chloride in four rain, and the pollution rate and induction rate of callus were 7%, 8% and 40%, 36%, respectively. The best sterilization method for leaves was 75% alcohol in 30 sec and 0.1% mercuric chloride in 7 min, and the pollution rate and induction rate of callus was 10% and 7%, respectively. These results could provide the basis on establishing the regeneration system and rapid propagation of Perilla frutescens （Linn） Britt.