EB病毒感染与系统性红斑狼疮相关性研究

Study on the relationship between Epstein-Barr virus infection and systemic lupus erythematosus

【目的】检测系统性红斑狼疮(systemic lupus erythematosus,SLE)患者及健康人外周血单个核细胞(PB-MCs)中Epstein-Barr病毒(epstein-barr virus,EBV)DNA拷贝量,探讨EBV感染与SLE之间的关系以及预防和治疗SLE的新途径。【方法】收集确诊SLE患者60例(49例为活动期,11例为稳定期)及健康对照32例,运用实时荧光定量PCR方法检测两组PBMCs中EBV DNA载量,同时分析60例SLE患者的部分临床化验指标与EBV感染之间的关系。【结果】SLE患者组与对照组PBMCs中EBV DNA检出率有统计学差异(χ2=4.217,P=0.040);SLE活动期与稳定期患者PBMCs中EBV DNA检出率没有统计学差异(χ2=0.000,P=1.000)。SLE患者中测出EBV DNA者,其病毒载量与SLE疾病活动评分(SLEDAI)之间的相关性没有统计学意义(r=0.332,P=0.267)。SLE患者根据检出EBV DNA与否分组后,两组的丙氨酸转氨酶(ALT)升高比较有统计学差异(χ2=6.314,P=0.012)。【结论】EBV感染与SLE相关;EBV感染可能参与SLE发病;EBV感染与SLE患者肝功能损害相关。

[ Objective] To investigate the relationship between EBV infection and SLE, and the method of prevention and treatment in SLE. [Methods] 60 （49 active vs. 11 inactive） patients with SLE and 32 normal controls were enrolled in the study. EBV DNA from PBMCs was measured with real - time fluorescent quantitative polymerase chain reaction （PCR） . We also analyzed whether the EBV Infection affect hepatic function, renal function, hematological system or other aspects of SLE patients. [Results] EBV DNA detection rate of SLE patients is higher than normal controls （X2 = 4.217, P = 0.040） . there was no difference between the active diseases and the inactive ones （ X2 = 0.000, P= 1.000） .The EBV DNA load of 13 SLE patients was not correlated with SLEDAI （ r =0.332, P =0.267） .13 of 60 SLE patients EBV DNA were detected, 47 were not. The levels of ALT was elevated in 6 of 13, while 5 of 47 was so, there was difference in the two groups （X2 =6.314, P =0.012） . [ Conclusions] There is association between EBV infection and SLE. EBV infection may also impair or aggravate hepatic function of SLE patients.