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农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究 被引量：21

Studies on Subcellular Localization of MpASR in Onion Epidermal Cells Mediated by Agrobacterium
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摘要 通过比较不同侵染液浓度、侵染时间、取材部位、预处理等条件下的转化效果,优化了农杆菌介导的洋葱鳞茎内表皮细胞转化系统,并且成功检测到大蕉ASR蛋白(MpASR)与GFP的融合蛋白在洋葱表皮细胞中的分布。 The effects of several factors on onion （Allium cepa L.） epidermal cells transformation were studied,including concentration of infection solution, infection time, parts of material and pre-treatment. The transformation system for onion epidermal cells mediated by Agrobacterium was established. The location of MpASR from Musa sapientum fused with GFP in onion epidermal cells was detected successfully.
作者 刘海燕 冯冬茹 刘兵 何炎明 王宏斌 王金发
机构地区 有害生物控制与资源利用国家重点实验室
出处 《热带亚热带植物学报》 CAS CSCD 北大核心 2009年第3期218-222,共5页 Journal of Tropical and Subtropical Botany
基金 广东省自然科学基金项目(7003637) 国家自然科学基金项目(30771235)资助
关键词 MpASR蛋白 亚细胞定位 洋葱 内表皮细胞 绿色荧光蛋白 农杆菌 MpASR Subcellular localization Onion Epidermic cell GFP Agrobacterium
分类号 Q943.2 [生物学—植物学]
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参考文献15

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二级参考文献27

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共引文献33

同被引文献233

引证文献21

二级引证文献43

热带亚热带植物学报
2009年 第3期

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