男性肥胖患者皮下脂肪组织PPARγ_2、leptin、TNF-α mRNA的表达与胰岛素抵抗

PPARγ_2,LEPTIN,TNF-α mRNA EXPRESSION OF SUBCUTANEOUS ADIPOSE TISSUE IN ABDOMEN OF OBESE MALES AND THEIR RELATIONSHIPS TO INSULIN RESISTANCE

目的:研究肥胖患者腹部皮下脂肪组织过氧化物酶体增殖物激活受体γ-2(PPARγ2)、瘦素(leptin)、肿瘤坏死因子-α(TNF-α) mRNA与胰岛素抵抗(IR)的关系。方法:应用RT-PCR凝胶成像半定量技术测定男性肥胖患者皮下脂肪组织PPARγ2 mRNA、leptin mRNA及TNF-α mRNA,以及空腹血清leptin、胰岛素(FINS)、空腹血糖(FBS),计算胰岛素抵抗指数(IRI),分析观察指标与肥胖之间的相关关系。结果:①肥胖组FINS、leptin、IRI、脂肪组织mRNA、leptin mRNA及TNF-α mRNA高于非肥胖组(P<0.05);②IRI与leptin mRNA、TNF-α mRNA、PPARγ2 mRNA、leptin、BMI、FINS成正相关(P<0.05);③以IRI为应变量,TNF-α mRNA、PPARγ2 mRNA、leptinmRNA、血清leptin、BMI为自变量做多元逐步回归分析,回归方程:Y=0.358+0.813PPARγ2mRNA。结论:①男性肥胖患者leptin及腹部皮下脂肪组织PPARγ2 mRNA、leptin mRNA、TNF-α mRNA较非肥胖者升高;②男性患者PPARγ2 mRNA与IR密切相关,可以反映IR的程度。

Objective：To study the expression of PPARγ2 mRNA,Leptin mRNA and TNF-α mRNA in subcutaneous adipose tissue of abdomen in obese males and their relationships to insulin resistance.Methods：Fasting serum levels of leptin,insulin,glucose were measured in 17 obese and 21 nonobese subjects. The mRNA expression of PPARγ2,leptin,TNF-α in subcutaneous adipose tissue in the abdomen was quantified in all of subjects by RT-PCR. Insulin resistance index（IRI） was calculated in Homeostasis model assessment HOMA）. Then the relationships between them were analyzed.Result：①The fasting serum levels of leptin,insulin and IRI were significantly higher in obese group than in nonobese group（FINS：26.13±13.14 mU/L vs 8.23±3.34 mU/L,P〈0.001; leptin：10.14±4.68 ng/mL vs 5.79±1.66 ng/mL,P〈0.01; IRI：5.43±6.11 vs 1.51±0.84,P〈0.001）. The mRNA expression of PPARγ2,leptin and TNF-α in the subcutaneous adipose tissue were significantly higher in obese group than in nonobese group（PPARγ2 mRNA：1.532±0.80 vs 0.96±0.45,P〈0.05; leptin mRNA：1.17±0.73 vs 0.80±0.20 P〈0.05; TNF-α mRNA：1.34±0.60 vs 0.75±0.34;P〈0.001）. ②IRI correlated with PPARγ2 mRNA,leptin mRNA,TNF-α mRNA levels,serum levels of leptin and insulin and BMI（P〈0.05）; ③PPARγ2 mRNA contributed to the degree of IRI,IRI=0.358＋0.813PPARγ2mRNA. Conclusion：The mRNA expression of PPARγ2,leptin,TNF-α in subcutaneous adipose tissue in abdomen were significantly higher in the male obeses.IRI was important factor in influence of the mRNA expression of PPARγ2.