青蒿素、青蒿琥酯抗皮肤瘢痕的体外实验研究

GROWTH INHIBITION OF FIBROBLASTS DERIVED FROM HUMAN BY ARTEANNUIN AND ARTESUNATE IN VITRO

目的:检测青蒿素和青蒿琥酯对人皮肤瘢痕成纤维细胞生长的影响,探索建立抗皮肤瘢痕膏剂的有效配方。方法:原代培养建立人皮肤瘢痕成纤维细胞系,MTT法和流式细胞仪检测细胞凋亡,计算增殖抑制率和凋亡指数。结果:浓度为0.206g/L和0.103g/L的青蒿素对人皮肤瘢痕成纤维细胞有明显的抑制作用;青蒿琥酯对人皮肤瘢痕成纤维有明显抑制作用,且呈浓度依赖性。浓度为0.206g/L的青蒿素和浓度为240、120mg/L的青蒿琥酯的凋亡率和坏死率较对照组高(P<0.01)。结论:青蒿素、青蒿琥酯可抑制体外培养的皮肤瘢痕成纤维细胞的生长,作为抗皮肤瘢痕外用膏剂的功效药物,值得进一步研究。

Objective： To investigate the effects of arteannuin and artesunate on the proliferation of fibroblasts derived from hypertrophic scars of human. Methods：Skin scar fibroblasts were cultured in vitro from human sources. MTT assay was used to examine the proliferation of the fibroblasts, and then the inhibition rate was calculated. Results：Arteannuin inhibited the proliferation of fibroblasts derived from human scar tissue at concentrations of 0. 206 g/L and 0. 103 g/L. Artesunate inhibited proliferation of fibroblast cells derived from human scar in dose and duration dependent manners. The apoptosis and necrotic rates of arteannuin and artesunate were higher than those in control group （ P 〈0.01）. Conclusion. Arteannuin, artesunate could inhibit the proliferation of fibroblasts in skin scars, and could be ingredient of ointment for skin scar inhibition and treatment.