目标起始密码子多态性(SCoT):一种基于翻译起始位点的目的基因标记新技术

SCoT:A Novel Gene Targeted Marker Technique Based on the Translation Start Codon

随着功能基因组学的发展,分子标记领域已开始从传统的随机或匿名性分子标记转向目的基因标记和功能性标记。目标起始密码子多态性(start codon targeted polymorphism,SCoT)标记是一种基于翻译起始位点的目的基因标记新技术,具有操作简单、重复性好等特点,根据ATG翻译起始位点侧翼保守序列来设计单引物,扩增产生偏向候选功能基因区显性多态性标记,适合不同层次实验室的不同领域的广泛应用,SCoT标记已开始在水稻和花生上得到运用。本文旨在介绍给研究者一种可以作为传统的RAPD、ISSR标记有效补充的目的基因标记新技术,希望其广泛的应用于生物多样性分析、遗传图谱构建、重要性状标记等方面。

With the development of functional genomics, the area of molecular markers has witnessed a shift from the so-called random DNA markers （RDMs） or anonymous markers to gene-targeted markers （GTMs） and functional markers （FMs）. Start codon targeted polymorphism （SCOT） is a novel gene targeted marker technique based on the translation start codon with the characteristics of simplicity and reproducibility. The primers for SCoT marker analysis were designed based on the short conserved region surrounding the ATG translation start （or initiation） codon. Conducting PCR amplification using the designed single primer can generate dominant polymorphic markers with a bias toward candidate functional genes region. The SCoT technique can be widely applied to different fields in different laboratories. SCoT polymorphism marker technique has been successfully applied in rice and peanut. The objectives of this article are to introduce a novel gene targeted molecular marker technique complementary to conventional RAPD, ISSR to researchers and to wish it to be widely exploited in the fields of genetic diversity, genetic map constructing and important agronomic traits tagging.