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陕西省31株木本植物炭疽菌的rDNA-ITS区序列分析 被引量:4

ITS-rDNA Sequence Analysis of Colletotrichum in Woody Plants in Shaanxi Province
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摘要 Thirty one isolates of Colletotrichum spp. from different woody plants in Shaanxi province were collected and identified by morphological characteristics. Genetic diversity and phylogeny among the 31 isolates were investigated by sequence analysis based on the rDNA-ITS sequence variation,and compared with 11 relevent sequences from GenBank. The results showed that there was rich genetic diversity in Colletotrichum species; and the sequences diversity index was 0.1957. All the Colletotrichum isolates were clustered into three groups at the bootstrap value of 94%. The Colletotrichum gloeosporioides isolates were clustered into the G1、G2 and G3,respectively. Some isolates of this species exhibited extensive genetic variability compared with the other specie isolates. There was also genetic difference among the isolates collected from the same host tree. It is reasonable to use complex species (Colletotrichum gloeosporioides) to refer to the woody Colletotrichum pathogens at present. Thirty one isolates of Colletotrichum spp. from different woody plants in Shaanxi province were collected and identified by morphological characteristics. Genetic diversity and phylogeny among the 31 isolates were investigated by sequence analysis based on the rDNA-ITS sequence variation, and compared with 11 relevent sequences from GenBank. The results showed that there was rich genetic diversity in Colletotrichum species; and the sequences diversity index was 0. 1957. All the Colletotrichum isolates were clustered into three groups at the bootstrap value of 94 %. The Colletotrichum gloeosporioides isolates were clustered into the G1, G2 and G3, respectively. Some isolates of this species exhibited extensive genetic variability compared with the other specie isolates. There was also genetic difference among the isolates collected from the same host tree. It is reasonable to use complex species ( Colletotrichum gloeosporioides ) to refer to the woody Colletotrichum pathogens at present.
出处 《林业科学》 EI CAS CSCD 北大核心 2009年第5期164-168,共5页 Scientia Silvae Sinicae
基金 国家自然科技资源共享平台(2005DKA21207-10) 长江学者和创新团队发展计划资助(IRT0748) 陕西省自然科学基金(陕C113)
关键词 炭疽菌属 RDNA-ITS 多样性 系统进化 Colletotrichum rDNA-ITS genetic diversity phylogeny
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