还原型谷胱甘肽对梗阻性黄疸大鼠肾脏线粒体保护作用的研究

Protective effect of reduced glutathione on renal mitochondria in rats with obstructive jaundice

目的:探讨氧自由基在梗阻性黄疸引发肾脏损害中的作用以及还原型谷胱甘肽对肾脏线粒体的保护作用。方法:将54只Wistar大鼠随机分为假手术对照组(A组),胆总管结扎组(B组),胆总管结扎+药物治疗组(C组)。采用胆总管结扎法建立大鼠梗阻性黄疸模型,C组采用还原型谷胱甘肽每天150mg/kg腹腔注射治疗21d。3组大鼠分别于术后第7、14、21天处死,每组每次处死6只。检测血清胆红素(BIL)、肌酐(Cr)、尿素氮(BUN)以及肾脏线粒体脂质过氧化产物丙二醛(MDA)和线粒体膜胆固醇含量的变化,并观察肾脏病理组织学改变。结果:B组及C组大鼠肾脏线粒体膜胆固醇及MDA含量高于A组(P<0.05),但C组增高的程度低于B组(P<0.05),并且病理改变也较轻。结论:氧自由基所引发的脂质过氧化作用是梗阻性黄疸引起肾脏损害的重要原因之一,应用还原型谷胱甘肽可减少脂质过氧化作用对肾脏的损害作用,对梗阻性黄疸时的肾脏损害具有保护作用。

Objective： To investigate the role of oxygen free radicals in renal dysfunction of rats with obstructive jaundice and the protective effect of reduced glutathione on renal mitochondria. Methods： Fifty-four Wistar rats were randomly divided into three groups： sham-operation control group （group A）, obstructive jaundice group （group B）, reduced glutathione treated group （group C）,The model of obstructive jaundice in rats was established by common bile duct ligation （CBDL）. In group C, reduced glutathione （150mg/kg.d） was given through abdominal cavity for 21 days. The rats were executed at 7th, 14th, and 21st day after operation respectively. The contents of serum BIL, Cr, BUN and the contents of renal mitochondria MDA and cholesterol were detected respectively, and the changes of renal histopathology were observed, iResults： The contents of renal mitochondria MDA and cholesterol in group B and group C were markedly higher than group A （P〈0.05）, and the contents of MDA and cholesterol in group C were much lower than group B （P〈0.05）, the changes of renal histopathology were minor in group C. Conclusion： Oxygen free radicals play an important role in the pathogenesis of renal lipid peroxidization injury in rats with obstructive jaundice. Reduced glutathione can protect renal mitochondria from the impact of lipid peroxidization injury.