猪SGCB基因多态性及其与胴体性状的关联分析

Association of the Polymorphism of Porcine SGCB Gene with Carcass Traits

本研究检测了大白猪、长白猪和梅山猪SGCB基因第4外显子上的G/A突变,并进行了多态性研究。采用改进的扩增受阻突变系统-PCR(ARMS-PCR)技术在3个不同猪种和"大白×梅山"F2代资源家系中进行遗传分析,结果表明,等位基因A和G在4个群体中分布平衡。对266头"大白×梅山"F2代资源家系中SGCB基因多态性与胴体性状进行关联分析。结果表明:该位点AA基因型对活体瘦肉率存在极显著影响(P<0.01),GG基因型对眼肌高存在极显著影响(P<0.01)。

The sequence of extron 4 of the porcine SGCB gene was obtained from Large White, Landrace and Meishan pigs. Sequence comparison revealed a A/G mutation which was detected by ARMS-PCR. The A and G alleles were balanced in the Large White, Landrace, Meishan pigs and Large White × Meishan F2 population. Association analysis between the polymorphism and carcass traits was performed in Large Whiten Meishan F2population with 266 pigs. The genotype AA had significant effect on average lean meat percentage（P〈0.01）, the genotype GG had significant effect on loin-muscle height（P〈0.01） in Large Whiten Meishan F2 population.