IL-2基因转移对卵巢癌细胞系SKOV3致瘤性的影响

The effect of IL 2 gene transfer on the tumorigenesis of ovarian cancer cell line SKOV3.

目的：探讨ＩＬ－２基因转移对卵巢癌细胞系ＳＫＯＶ３致瘤性的影响。方法：通过贴壁集落形成检查、软琼脂集落形成检查和裸鼠皮下成瘤试验，测定ＩＬ－２基因转移对卵巢癌细胞系ＳＫＯＶ３致瘤性的影响。结果：ＳＫＯＶ３、ＳＫＯＶ３／Ｎｅｏ和ＳＫＯＶ３／ＩＬ－２细胞贴壁集落形成率、软琼脂集落形成率分别为５２％、４５％、０．７％和３１．４％、２８．５％、２．４％，ＳＫＯＶ３／ＩＬ－２细胞所形成的集落内细胞少，结构松散；裸鼠皮下成瘤实验显示，３组细胞皮下成瘤率分别为１００％（１２／１２）、９１．７％（１１／１２）及５６．３％（９／１６）；成瘤潜伏期平均分别为６．７±０．９ｄ、１１．４±４．０ｄ、１５．１±６．４ｄ，肿瘤终体积平均分别为１６０４．３±１４６９．４ｍｍ３、１１３０．３±１１５９．７ｍｍ３及２３３．３±１２６．４ｍｍ３；ＳＫＯＶ３／ＩＬ－２细胞皮下瘤增殖较ＳＫＯＶ３／Ｎｅｏ和ＳＫＯＶ３细胞明显缓慢（Ｐ＜０．００１）。镜下见ＳＫＯＶ３及ＳＫＯＶ３／Ｎｅｏ组肿瘤呈浸润性生长，并于皮下和肌肉间形成转移病灶，ＳＫＯＶ３／ＩＬ－２组肿瘤呈假包膜状生长，瘤内见大量单个核细胞浸润。结论：ＩＬ－２基因转移可降低细胞系ＳＫＯＶ３的致瘤?

Objective:To study the effect of IL 2 gene transfer on the tumorigenesis of ovarian cancer cell line SKOV3.Methods:Colony forming examination,soft agar colony forming examination,and subcutaneous tumor forming test in nude mice were used to detect about the mentioned effects.Results:The average colony forming efficiency of SKOV3,SKOV3/Neo and SKOV3/IL 2 cells in colony forming examination and soft agar colony forming examination were 52%,45%,0.7% and 31.4%,28.5%,2.4% respectively.The average latency and tumorigenesis of subcutaneous tumor forming in nude mice were 6.7±0.9d,11.4±4.0d,15.1±6.4d and 100%(12/12),91.7%(11/12),56.3%(9/16)respectively.The average end tumor volumes were 1604.3±1469.4mm 3,1130.3±1159.7 mm 3 and 233.3±126.4mm 3 respectively,SKOV3/IL 2 grew more slowly than that in the control group.Histologic study demonstrated that SKOV3 and SKOV3/Neo were more aggressive than SKOV3/IL 2 cells and invasive focuses were formed.There was large amount of mononuclear cells infiltrated into the tumor of SKOV3/IL 2 cells.Conclusion:hIL 2 gene modification can reduce the tumorigenesis of ovarian cancer cell line SKOV3 and induce the infiltration of mononuclear cells into the subcutaneous tumor of nude mice.