气相色谱法用于吡虫啉农药中毒血清分析

Gas chromatography for analysis of imidacloprid in the poisoning serum

【目的】建立快速检测血清中吡虫啉的气相色谱法。【方法】采用玻璃填充柱（2．0m×3．2mm，ID），填充3％OV．17＋10％DC．200（1：1），氮磷检测器，恒温操作，载气为高纯氮，乙酸乙酯做萃取剂。【结果】吡虫啉线性范围在0．02—0．50μg/ml，平均回收率87．5％-102．5％；日内、日间RSD：3．49％～4．90％（n=5），最低检出浓度：0．010μg/ml。回归方程：Y=7．628x＋0．0835，r=0．9996。【结论】玻璃填充柱分离血清中的吡虫啉，方法简便快速，灵敏度高，分离杂峰少且容易判定，可以满足临床急救需要。

[Objective] To establish the gas chromatography for detecting the imidacloprid in serum sample. [Methods] Glass packed column （2.0 m x 3.2 mm, ID）, filled 3% OV-17 ＋ 10% DC-200 （1： 1）, nitrogen and phosphorus detec- tor, thermostat operation, high-purity nitrogen as carrier gas, ethyl acetate as the extractant. [Results] The linear range ofimidacloprid is 0.02 - 0.50 μg/ml, the average recovery rate of 87.5% - 102.5%; days, day RSD： 3.49% - 4.90% （n = 5）, the minimum detectable concentration： 0. 010μg/ml. Regression equation： y = 7.628x ＋0.0835, r = 0.9996. [Conclusions] To separate from the imidacloprid in serum sample by glass column, the method is easy and quick, high sensitivity, less separation miscellaneous, easy to meet the needs of first-aid.