二氢埃托啡自动静脉给药对大鼠免疫功能的影响

EFFECT OF DIHYDROETORPHINE ON IMMUNE FUNCTIONS IN RATS TREATED BY AUTOMATICAL INTRAVENOUS INJECTION

本研究采用计算机实时控制的定时自动静脉给药法，７２ｈ快速致使大鼠身体依赖性形成。各组戒断症状分值为：ＮＳ组１１．０±ｓ２．９；吗啡组８１．０±ｓ８．６；ＤＨＥ组４１．０±ｓ４．４，表明大鼠已形成明显身体依赖性（Ｐ＜０．００１）。在该模型基础上，利用３Ｈ－ＴｄＲ掺入法测得ＤＨＥ明显抑制大鼠淋巴细胞增殖反应。各组刺激指数为：８０．６９±ｓ１．７６；４９．０４±ｓ１．１０和３０．７７±ｓ２．１４（Ｐ＜０．００１）。利用ＭＴＴ法测ＤＨＥ也能抑制大鼠ＩＬ－２活性，各组Ａ５７０ｎｍ值为：０．３９±ｓ０．０３；０．３１±ｓ０．０４和０．２６±ｓ０．０４（Ｐ＜０．０１）。结果表明ＤＨＥ自动静脉给药明显抑制大鼠免疫功能。

Computer controlled automatical intravenous injection was adopted to establish physical dependence in DHE treated rats. The withdrawal scores of 2 opiate-dependent rat groups were: DHE group 41.0±s 4.4; Morphine group 81.0±s 8.6. Both showed significant differences compared with that of NS group (11.0±s 2.9, P< 0.001).Based on this modal, effects of DHE on immune functions were examined. DHE significantly suppresses the Con-A activated splenic lymphocyte proliferations in rats. The stimulation indexes were: DHE group 30.77±s 2.14; Morphine group 49.04±s 1.10.Both showed significant differences compared with that of NS group (80.67±s 1.76, P< 0.001).DHE can also suppress the activity of IL-2 in the supernatant of Con-A activated splenocytes in rats. The values of A570nm were: DHE group 0.26±s 0.04; Morphine group 0.31±s 0.04. Both showed differences compared with that of NS group (0.39±s 0.03, P<0.01).The results indicate that DHE has powerful immunosuppressive effects in rats; furthermore, its effects are at least as 1000 times as those of morphine at the same dose.