荧光法测定血液中脂质氧化损伤标志物及抗氧化物的研究与临床应用

Investigation on Pinworm Infection of Children in the Urban and Rural Kindergarten in Luoyang City

目的：探讨血液中脂质氧化损伤标志物及抗氧化物的检测方法。方法：对荧光法测定血液过氧化脂质（ＬＰＯ）、水溶性荧光物质（ＷＳＦＳ）、还原型谷胱甘肽（ＧＳＨ）、谷胱甘肽过氧化物酶（ＧＳＨＰｘ）和维生素Ｅ（ＶｉｔＥ）的实验条件、测定步骤、激发波长和发射波长进行研究，并进行系统的方法学评价。结果：改进或建立了血液ＬＰＯ、ＷＳＦＳ、ＧＳＨ、ＧＳＨＰｘ和ＶｉｔＥ的荧光测定法，并应用于临床。结论：这些方法简便、快速、准确、灵敏、稳定、经济，适合临床应用。

Objective: Pinworm infection of children in urban and rural areas in Luoyang City was investigated in order to provide a means for prevention and cure of the disease.Methods: By way of cellophane swab method,it was stuck to the anus surrounding of the subjects,and then taken away from it to be sent for examination of ova under a microscope.Results: Infection rate of pinworm in those children in urban and rural areas were 21.73% and 65.57%,respectively with markable difference (P<0.01).Infection rate of male and female children was 46.45% and 43.98%,respectively,without markable difference (P>0.05).The infection rate of children at different ages in urban district was from 5.71% to 28.00%,the highest rate being 28.00% at the group aged 4.The infection rate of those in rural area was from 62.70% to 67.20%.There was no significant difference in the groups of different ages (P>0.05).Conclusion: The infection rate in rural area was obviously higher than that in urban district,and there was no marbable difference between male and female children.