VR1与CGRP和IB4在大鼠结状神经节和岩神经节内的共存

Co-localization of vanilloid receptor subtype 1 with calcitonin gene-related peptide and isolectin B4 in nodose and petrosal ganglia of the rat

应用免疫荧光组织化学三标方法结合激光共聚焦显微镜技术研究了辣椒素受体(VR1)在大鼠舌咽神经和迷走神经内脏感觉神经节结状神经节(NG)和岩神经节(PG)内的表达,以及与降钙素基因相关肽(CGRP)、植物凝集素(IB4)的共存。结果显示:VR1在NG和PG内中、小型神经元胞体和神经纤维存在广泛的表达。许多VR1阳性神经元呈IB4阳性或与CGRP共存。在NG,CGRP阳性神经元数量较少,约有71.4%±3.8%VR1阳性神经元与IB4共存,只有7.1%±1.2%VR1阳性细胞与CGRP共存。PG内CGRP阳性神经元胞体数量较多,有55.7%±3.1%VR1阳性神经元与IB4共存;有38.7%±2.7%VR1阳性细胞同时呈CGRP阳性。两个神经节内IB4/CGRP双标神经元或VR1/CGRP/IB4三标神经元数量稀少。上述结果提示舌咽神经和迷走神经内脏感觉神经节内存在VR1/IB4和VR1/CGRP两种不同的与伤害性刺激相关的VR1阳性神经元亚群。

The expression of vanilloid receptor subtype 1 （ VR1 ） and its co-localization with calcitonin gene-related peptide （CGRP） and the isoleetin B4 （IB4） in the glossopharyngeal and vagal visceral sensory ganglia, nodose （NG） and petrosal ganglia （PG） of the rat, were studied by triple fluorescent immunohistochemistry combined with laser scanning eonfocal microscopic technique. The results showed that extensive expression of VR1 was found in neuronal cell bodies and fibers in these two ganglia. Most of VR1 immunoreaetive neurons were middle and small in size. Numerous VR1 immunoreactive neuronal cell bodies expressed the IB4 reactivity or co-localized with CGRP. In the NG, a small number of CGRP immunoreaetive neurons was observed. There were about 71.4% ＋ 3.8% VR1 immunoreac tire neuronal cell bodies showed IB4 reactivity and 7.1% ＋ 1.2% VR1 immunopositive neurons also showed immunoreactivity for CGRP. In the PG, numerous CGRP immunoreactive neurons were observed. About 55.7% ± 3.1% VR1 immunoreactive neurons showed IB4 reactivity and 38.7% ±2.7% VR1 immunopositive neurons co-localized with CGRP. IB4/CGRP double-labeling and VR1/CGRP/IB4 triple-labeling cell bodies were rare in these two ganglia. These findings suggest that there are VR1/IB4 and VR1/CGRP double-labeling neurons, both of the two different VR1 immunoreactive neuronal subgroups might be served for nociception in glossopharyngeal and vagal visceral sensory ganglia.