应用改变的Golgi-Cox染色方法检测纹状体内神经元的结构与形态(英文)

A modified Golgi-Cox staining method for study the structure and morphology of neurons in the striatum

为了改变传统的Golgi-Cox染色方法,使其在纹状体神经元形态与结构的研究中更加稳定和有效,本实验将昆明小鼠随机分为两组,一组采用传统的Golgi-Cox染色法,另一组采用改良的Golgi-Cox染色法。改良方法在传统方法的基础上,改变了几个关键环节,包括溶液的配制、固定、包埋、切片和定影等,然后对两种方法进行统计学比较和分析。在所有的数据采集工作完成之前,切片上的标记是封闭的。通过统计学分析比较,发现改良方法能够稳定地显示更多的树突分支(增加50%)、树突棘(增加63%)和胞体(增加一倍)。改良的Golgi-Cox染色方法比传统的Golgi-Cox染色方法更加稳定和敏感,在纹状体神经元树突和树突棘形态与结构研究中是一种可靠的技术方法。

In order to modify the traditional Golgi-Cox staining method to make it more effective and stabilized in studying the structure and morphological changes of neurons in striatum, Kunming mice were randomly divided into two groups for the comparision in the present study. One group conformed to guideline of traditional Golgi-Cox method, while the other group followed our modified protocol. The traditional Golgi-Cox method was modified in several key steps, including the preparing of materials and solutions, fixing, embedding, sectioning and development. The results of these two methods were compared and analyzed. No codes are broken until all the data collection has been completed. Comparing to the traditional Golgi-Cox staining method, our modified method provides more reliable staining for fine branches （50% increased） , spines （63% raised） and somas （multiply doubly）. The modified Golgi-Cox technique is more reliable and sensitive than the traditional Golgi-Cox staining method and is a good technique to study the structure of dendrites and dendritic spines of neurons in striatum.