慢传输型便秘肠神经系统一氧化氮合酶和P物质的分布意义

Significance of nitric oxide synthase and substance P distribution in enteric nervous system of slow transit constipation *

目的研究结肠一氧化氮（ＮＯ）和Ｐ物质（ＳＰ）在慢传输型便秘（ＳＴＣ）发病中的作用．方法应用一氧化氮合酶（ＮＯＳ）和ＳＰ的兔多克隆抗体，对手术切除的１５例ＳＴＣ患者（男２例，女１３例，年龄２６岁～６８岁）和１１例对照组患者乙状结肠标本进行免疫组化染色和半定量分析．结果ＳＴＣ患者乙状结肠肌间神经丛ＮＯＳ免疫反应性明显升高（χ２＝１５６３，Ｐ＜００１），而ＳＰ免疫反应性明显降低（χ２＝１３４４，Ｐ＜００１）；粘膜下神经丛ＮＯＳ及ＳＰ免疫反应性与对照组相差不显著．结论ＳＴＣ结肠神经系统ＮＯＳ和ＳＰ免疫反应性的异常变化。

AIM To investigate the role of enteric nervenitric oxide (NO) and substance P (SP) in slow transit constipation (STC). METHODS By using immunohistochemistry and semiquantitative analysis technique, the distribution of NOS and SP in sigmoid colon of 15 patients with STC (2 males, 13 females, with an age range of 26 to 68 years) and 11 control patients were investigated. RESULTS The immunoreactivity of nitric oxide synthase (NOS) was increased (χ 2=15 63, P <0 01),while that of SP was decreased significantly (χ 2=13 44, P <0 01) at the myenteric plexus of sigmoid colon of STC. The immnoreactivity of NOS and SP had no difference compared with control group at submucous plexus. CONCLUSION Abnormal NOS and SP immunoreactivity in colonic enteric nervous system could be the neuropathologic bases of STC.