鼠自身免疫性肝炎模型的建立

Establishment of experimental autoimmune hepatitis model in rats

目的探索鼠实验性自身免疫性肝炎（ＥＡＨ）模型的建立．方法用♀重１３０ｇ～１６０ｇＷｉｓｔａｒ大鼠分成８组，♀重２５ｇ～３５ｇＢＡＬＢ／ｃ小鼠分成２组（每组ｎ＝６）．Ⅰ组为ＰＢＳ对照组；Ⅱ组为ＣＦＡ；Ⅲ组为大鼠Ｓ１００２５ｍｇｉｐ；Ⅳ组为大鼠Ｓ１００２５ｍｇ加ＣＦＡｉｍ；Ⅴ组为大鼠Ｓ１００５ｍｇ加ＣＦＡｉｐ；Ⅵ组为大鼠Ｓ１００１０ｍｇ加ＣＦＡｉｐ；Ⅶ组为大鼠Ｓ１００２５ｍｇ加ＣＦＡｉｐ；Ⅷ组为小鼠Ｓ１００２５ｍｇ加ＣＦＡｉｐ组；Ⅸ组为大鼠Ｓ１００２５ｍｇ加ＣＦＡｉｐ给小鼠组；Ⅹ组为小鼠Ｓ１００２５ｍｇ加ＣＦＡｉｐ给小鼠组．观察生存率、肝功能、免疫球蛋白、肝脏的病理改变．结果用同种肝抗原Ｓ１００辅以福氏完全佐剂腹腔免疫Ｗｉｓｔａｒ大鼠可诱导ＥＡＨ模型，该法形成率高（９８％）、死亡率低（２％）．病理检查显示典型的ＥＡＨ病变．ｗｋ５血清ＡＬＴ为２００ＩＵ／Ｌ、ＩｇＧ为５５ｇ／Ｌ．

AIM To explore the establishment of experimental autoimmune hepatitis (EAH) model in rats. METHODS Forty eight female Wistar rats weighing 130g-160g were divided into eight groups ( n =6). Twelve female BALB/c mice weighing 25g-35g were divided into two groups ( n =6). Group Ⅰ, PBS control; group Ⅱ, CFA; group Ⅲ, rat S 100 2 5mg ip ; group Ⅳ, S 100 2 5mg with im CFA; group Ⅴ, rat S 100 5mg with CFA ip ; group Ⅵ, rat S 100 10mg with ip CFA; group Ⅶ, rat S 100 2 5mg with ip CFA; group Ⅷ, mice S 100 2 5mg with ip with CFA; group Ⅸ, rat S 100 2 5mg with ip CFA into mice; and group Ⅹ, mice S 100 2 5mg with ip CFA. The survival rates, liver function and immunoglobulin tests and histologic examinations were observed. RESULTS EAH model was induced by intraperitoneal immunization with antigen liver homogenate (S 100) in complete Freund adjuvant with a high success (98%) and low mortality rate (2%). Pathological examinations indicated typical histopathological changes of EAH. Serum ALT was 200IU/L and IgG 5 5g/L in the 5th week. CONCLUSION The typical experimental autoimmune hepatitis model can be induced by S 100 with CFA.