青老年人胃癌p53及增殖细胞核抗原表达的对比

Comparative studies on p 53 and PCNA expressions in gastric carcinoma between young and aged patients

目的通过对老人青年胃癌中ｐ５３及增殖细胞核抗原（ＰＣＮＡ）表达的研究，探讨青老年人胃癌临床表现的分子生物学异同．方法应用过氧化物酶标记的链霉卵白素（ＳＰ）染色法对６５例胃癌（青年组３５例，≤３５岁；老年组３０例，≥６０岁）进行ｐ５３蛋白及ＰＣＮＡ的免疫组织化学染色．并对胃癌细胞中ＰＣＮＡ阳性颗粒进行图象分析．结果ｐ５３和ＰＣＮＡ在青年人胃癌中的阳性率分别为６５７％和９４３％，均显著高于老年人（２６７％和５００％，Ｐ＜００１）．青年人胃癌中ＰＣＮＡ阳性颗粒平均光密度及积分光密度均显著高于老年人（Ｐ＜００５）．结论ｐ５３及ＰＣＮＡ的高表达可能是青年人胃癌恶性度高，进展快。

AIM To explore the molecular biological difference between young and aged patients in p 53 and proliferating cell nuclear antigen (PCNA) expression in gastric carcinoma with immunohistochemistry. METHODS By streptavidin/peroxidase (SP) method, 65 patients with gastric carcinoma including 35 young (≤35 years) and 30 aged (≥60 years) patients were examined immunohistochemically for p 53 products. The mean optical density (MOD) and the integral optical density (IOD) of positive cells for PCNA were tested with image analysis system. RESULTS p 53 and PCNA positive expression rates in young patients with gastric carcinoma (65 7% and 94 3%) were significantly higher than those in their aged counterparts (26 7%, 50 0%, P <0 01). The MOD and IOD of PCNA in young patients were dramatically stronger than those in aged patients ( P <0 05). CONCLUSION The high positive expression of p 53 and PCNA may be caused by the higher malignancy, more rapid progress and poorer prognosis in young patients with gastric carcinoma.