原发性肝细胞癌组织中P16蛋白表达的意义

Significance of P16 protein expression in hepatocellular carcinoma tissues

目的研究原发性肝细胞癌中Ｐ１６蛋白的表达程度与转移及预后的关系．方法应用免疫组织化学ＬＳＡＢ法对４５例原发性肝细胞癌组织及癌旁组织进行Ｐ１６蛋白表达的检测，并结合临床资料进行分析．结果肝癌肿瘤区及癌旁区Ｐ１６蛋白阳性表达率分别为４００％和８４４％，两组比较，有显著性差异（Ｐ＜００５）．Ｐ１６蛋白阳性表达率在无转移的肝癌中为６０５％，有转移组为２００％；高、中和低分化的肝癌分别为５００％，２７８％和２２２％．故Ｐ１６蛋白阳性表达在肝癌患者高分化与中和低分化比较有显著性差异（Ｐ＜００５），转移与非转移组比较有极显著性差异（Ｐ＜００１）．结论Ｐ１６蛋白表达与肝细胞癌增殖。

AIM To study the relation of P16 protein expression in hepatocellular carcinoma (HCC) with metastasis and prognosis. METHODS P16 protein expression was studied in 45 patients with HCC by using immunohistochemical LSAB method and compared with clinical materials. RESULTS P16 protein positive expression in the tumors and their neighboring regions was 40 0% and 84 4%, with a significant difference ( P <0 05). The positive expression of P16 protein was 20 0% in the carcinoma with metastasis and 60 5% without metastasis. P16 protein expression in highly, moderately and poorly differentiated HCC was 50 0%, 27 8% and 22 2%, with an obvious difference between patients with highly differentiated and poorly differentiated HCC ( P <0 05) , between groups with and without metastasis ( P <0 01) . CONCLUSION P16 protein expression is closely related to proliferation, malignant potential and prognosis of HCC.