尿紧张素Ⅱ检测及表达与子痫前期孕妇的关系研究

The examination and the expression of urotensin Ⅱ and their relationship with pre-eclampsia

目的通过子痫前期孕妇和新生儿脐血尿紧张素Ⅱ(UⅡ)检测和UⅡmRNA在胎盘组织的表达水平,探索其在子痫前期发生、发展中的作用。方法1)应用ELISA方法对40例子痫前期患者及20例同期正常妊娠孕妇(健康对照组)外周血和新生儿脐血进行血清UⅡ测定。2)同时用RT-PCR检测胎盘组织中UⅡmRNA的表达水平。结果1)子痫前期组血清UⅡ浓度明显高于健康对照组,差异有统计学意义(P<0.05)。2)胎盘组织UⅡmRNA表达子痫前期组明显高于健康对照组,差异有统计学意义(P<0.05)。结论UⅡ在胎盘组织缺血缺氧和动脉粥样硬化的发生中起重要作用。

Objective To explore the effect of urotensinⅡ （U Ⅱ ） in occurrence and development of pre-eclampsia through the examination of U Ⅱin pre eclampsia pregnant woman and newborn cord blood and the expression of U Ⅱ mRNA in placenta. Methods 1） The serum U Ⅱ of peripheral blood and newborn cord blood was determined in 40 pre-eclampsia patients and 20 normal pregnant women （control group） by using ELISA method. 2） The U Ⅱ mRNA expression level in placenta tissue was simultaneously examined with RT-PCR method. Results The concentration of serum U Ⅱ in preeclampia patients was obviously higher than that in control group （P〈0. 05）. 2） The UⅡ mRNA expression level in placenta tissue in pre-eclampsia patients was obviously higher than that in control group （P〈0.05）. Conclusion U Ⅱ plays an important role in development of ischemia,anoxemia and atherosclerosis of placenta tissue.