转化生长因子-β和碱性成纤维细胞生长因子在腹股沟疝患者腹横筋膜中的表达

The expression of transforming growth factor-β and basic fibroblast growth factor in transversalis fascia of patients with inguinal hernia

目的观察转化生长因子（TGF）-β和碱性成纤维细胞生长因子（bFGF）在原发腹股沟疝患者腹横筋膜中的表达。方法采用免疫组织化学方法测定实验组（直疝组40人；斜疝组40人）及非疝对照组（20人）腹横筋膜中的TGF—β、bFGF的表达情况。结果TGF-β在直疝组多表现为局灶染色或弥漫性淡染（65．0％），但其在斜疝组及对照组多表现为弥漫性深染（42．5％；45．0％），TGF-β在直疝组与对照组之间的差异有统计学意义（P〈0．05）；而斜疝组与对照组之间的差异无统计学意义（P〉0．05）；bFGF在直疝组、斜疝组、对照组几乎都表现为局灶染色或散在淡染（85．0％、77．5％、65．0％），三组之间的差异无统计学意义（P〉0．05）。结论TGF—β的低表达影响了腹横筋膜的强度并进一步影响了腹股沟直疝的发生、发展，但其与斜疝无明显相关；bFGF与腹股沟疝的发生、发展无明显相关。

Objective To investigate the expression of TGF-β and bFGF in the transversalis fascia of patients with primary inguinal hernia. Methods All samples were obtained from transversalis fascia of patients with original inguinal hernia （40 direct hernias;40 indirect hernias） , and those from 20 volunteers without hernia disease served as controls. All samples were immunohistochemically labeled by anti- TGF-β and anti-bFGF. Results The expression of TGF-β in direct hernias was focal or diffuse but light, while it was diffuse and heavy in controls and indirect hernias. The expression of TGF-β in direct hernias was lower than that of controls （ P 〈 0.05 ）, but there was no statistically significant difference between indirect hernias and controls （P 〉0.05）. However,the expression of bFGF was diffuse but light in all the three groups, and there was no statistically significant difference between any of the groups （ P 〉 0.05 ）. Conclusion The lower expression of TGF-β weakens the transversalis fascia,and then influences the development of direct hernia, but maybe it has nothing to do with indirect hernia. The similar expression of bFGF suggests it has no relationship with inguinal hernia.