摘要
目的观察转化生长因子（TGF）-β1及其Ⅰ型受体在两种大鼠肺动脉高压模型中表达的变化。方法36只Spargue—Dawley大鼠，对照组、分流型和药物型肺动脉高压模型各12只，使用原位杂交方法观察TGF—β1及其Ⅰ型受体在肺小动脉表达的定位，并使用逆转录一聚合酶链反应（RT—PCR）法观察两者mRNA转录量的变化。结果大鼠分流型与药物型肺动脉高压模型中TGF—β1及其Ⅰ型受体mRNA主要分布于肺小动脉内皮细胞和外膜成纤维细胞，两种模型中TGF—β1（1．17±0．09、1．09±0．07）及其Ⅰ型受体（0．80±0．06、0．93±0．02）mRNA转录均较对照组明显增加，三组间均数比较差异有统计学意义（P〈0．05）。结论TGF-β1信号参与了肺动脉高压肺血管重塑的过程，但在不同的模型中作用机制不同。
Objective To compare the different expression of TGF-β1 and TGF-βR1 in two types of pulmonary artery hypertension model of rats. Methods Thirty-six Spargue-Dawley rats were divided into 3 groups （control, carotid artery-jugular vein shunt pulmonary hypertension model and monocrotalin-in- duced pulmonary hypertension model）. Each group had 12 rats. TGF-β1 and TGF-βR I localization was evaluated by in situ hybridization, and mRNA transcription was detected by RT-PCR. Results The locali- zations of TGF-β1 and TGF-13R I in two models were endothelial cells and extima fibroblast cells of pulmonary arteries, respectively. The expression of TGF-β1 （ 1.17 ± 0.09,1.09 ±0. 07） and TGF-βR I mRNA （0.80± 0.06,0.93± 0.02） was increased in both two models. There was significant difference in multiple comparison of three groups （P 〈 0.05）. Conclusion TGF-β1 signaling is involved in pulmonary vascular remodeling of pulmonary hypertension process, and its mechanisms in two pulmonary artery hypertension models are different.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第6期767-769,共3页
Chinese Journal of Experimental Surgery
基金
广东省科技计划基金资助项目（2008B030301076）
