特发性高草酸尿症大鼠肝脏差异蛋白质的筛选

Differentially expressed proteins in the liver of rats with idiopathic hyperoxaluria

目的寻找特发性高草酸尿症大鼠肝脏组织差异表达的蛋白质。方法取3只雄性特发性高草酸尿症大鼠和3只正常对照雄性大鼠，分别取其肝脏组织约300mg后提取其中的总蛋白。以荧光差异显示凝胶电泳（DIGE）技术分离肝脏中的全部蛋白质，用基质辅助激光解吸电离飞行时间质谱技术（MALDI—TOFMS）和DeCyderTMv6．5软件进行蛋白质的分析和鉴定。结果总共筛选出21个差异表达蛋白质点，其中11个蛋白质点在特发性高草酸尿症组表达上调，10个蛋白质点下调。最终有18个差异蛋白质点被鉴定确认。结论筛选出的这些差异蛋白质可能与特发性高草酸尿症有关。

Objective To investigate differentially expressed proteins in the liver of rats with idiopathic hyperoxaluria. Methods three male rats with idiopathic hyperoxaluria served as study group, and 3 normal ones as controls. The liver of rats with idiopathic hyperoxaluria and the normal liver tissue were examined by two-dimensional gel electrophoresis （2-DE） after labeled with CyDye DIGE Fluors Cy3, Cy5 and Cy2. Intensity changes of protein spots detected with statistical significance were identified by MALDI- TOF MS. Results Intensity changes of 21 spots were detected with statistical significance. Eleven protein spots of them were up-regulated, and the remaining 10 down-regulated. And 18 of them were identified by MALDI-TOF MS successfully. Conclusion DIGE is a useful technique for differential expressed proteins screening and analysis in the liver of rats with idiopathic hyperoxaluria. Pdia3, ALDH, TTR, etc. differently expressed proteins may be key proteins for idiopathic hyperoxaluria.