摘要
依据马铃薯Y病毒基因组序列,设计合成了1对引物,以从山西省获得的带毒马铃薯叶片总RNA为模板,通过RT-PCR扩增得到852 bp的基因片段并进行了序列测定。BLAST分析表明,该DNA序列与PVY N:O株系pl基因序列相似性最高可达99%。
A pair of specific primers was designed based on the gene sequence of potato which amplified a 852 bp DNA fragment from the total RNA of potato leaves infected collected from Shanxi province by RT-PCR. Sequence analysis showed that the DNA amplified had 99% similarity with that of pl gene of PVY strain N：O. virus Y, by PVY fragment
出处
《河南农业科学》
CSCD
北大核心
2009年第5期116-118,共3页
Journal of Henan Agricultural Sciences
基金
河南省教育厅自然科学基础研究项目(2006210003)
河南工业大学校科研基金项目(07XJC008)
