慢生型分枝杆菌体外抗菌药物敏感性试验研究

Drug susceptibility of slowly growing mycobacteria in vitro

目的:测定临床慢生型分枝杆菌对抗菌药物的敏感性。方法:采用美国临床和实验室标准协会M24-A液基微量稀释法,测定慢生型非结核分枝杆菌对8种抗菌药物的敏感性。结果:3株临床海鱼分枝杆菌的MIC值范围:阿米卡星均为1 μg/mL;多西环素1～8 μg/mL;克拉霉素0.5～2 μg/mL;利福平0.25～0.5 μg/mL;磺胺甲基异噁唑4～32 μg/mL。1株临床胞内分枝杆菌对克拉霉素敏感,MIC≤0.25 μg/mL。3株临床瘰疠分枝杆菌的MIC值:阿米卡星2～8 μg/mL;多西环素≥32 μg/mL;克拉霉素0.25～2 μg/mL;利福平0.25～4 μg/mL;磺胺甲基异噁唑≤1～4 μg/mL;乙胺丁醇2～32 μg/mL;异烟肼0.5～2 μg/mL;链霉素4～8 μg/mL。结论:临床慢生型非结核分枝杆菌对8种常用抗菌药物的敏感性存在差异。

Objective： To test the susceptibility of slowly growing mycobacteria to antimicrobial agents. Method： Clinical laboratory standard institute （CLSI） broth micmdilution method M24- A was applied to test the susceptibility of slowly growing mycobacteria to amikacin, doxycycline, clarithromycin, rifampin, sulfamethoxine, ethambutol, isoniazid and streptomycin. Results： MIC ranges of 3 strains of M. mar/num were： amikacin 1μg/mL, doxycycline 1 - 8μg/mL, clarithromyein 0.5 - 2μg/mL, rifampin 0.25 - 0.5μg/mL and sulfmnethoxine 4 - 32μg/mL. The clinical M. intracellulare was sensitive to clarithromycin with a MIC≤0.25 μg/mL. For 3 strains of M. scrofulaceurn, the MIC were： amikacin 2 - 8μg/mL, doxycycline ≥ 32μg/mL, clarithromycin 0. 25 ～ 2 μg/mL, rifampin 0. 25 - 4μg/mL, sulfamethoxine ≤ 1 - 4μg/mL, ethambutol 2 ～ 32 μg/mL, isonicid 0.5 - 2μg/mL and streptomy- cin 4 - 8μg/mL. Conclusion： The sensitivity of slowly growing mycobacteria varies from stain to stain.