超生理剂量维生素D3致小鼠动脉硬化及机制

Large Dose of Vitamin D3 Inducing Arteriosclerosis in Mouse

目的探讨超生理剂量维生素D3致小鼠动脉硬化及其机制。方法SPF级KM小鼠40只,随机分为对照组、超生理剂量维生素D3组和常量维生素D3组。超生理剂量维生素D3组给予小鼠40万u/(?.d)维生素D3灌胃诱导小鼠动脉硬化,连续给药3次,观察42d,取血管标本制作主动脉病理切片,Vonkossa染色计算钙化面积百分比,离子型发射光谱仪检测血管钙、磷含量。结果超生理剂量维生素D3组小鼠主动脉中膜严重钙化,动脉中膜平滑肌细胞表型改变为类软骨细胞,血管钙化面积百分比显著高于对照组(P<0.01),血管钙、磷含量较对照组均升高(P<0.05)。结论超生理剂量维生素D3可致小鼠主动脉硬化,机制与其诱导血管平滑肌细胞发生类软骨样细胞改变,同时导致大量钙、磷在血管内沉积有关。

Aim To study arteriosclerosis induced by large dose of vitamin D3 （VitD3） and its mechanism in mouse. Methods Forty Kunming mouse were randomly divided into three groups including control group, model group and normal VitD3 group, with sixteen in the forner two groups, in which male and female were eight equally and eight in the last group. Then mouse in model group were given forty units of VitD3 for three days and observed for forty- two days. Sections were made for aortic arch and stained by hematoxylin and eosin, thoracic aortawere stained by Von ko- ssa and the percentage of calcificational areas was counted, the content of Ca and P was measured by ICP. Results The media of aorta was seriously calcified in VitD3 model group compared with control group, phenotype of smooth muscle cell in media of aorta have changed to another phenotype which was suspected for chondrocyte, percentage of calcificational areas in model group was remarkably higher than in control group （P〈0.01）. The content of Ca and P for abdominal aorta was higher in model group than in control group （P〈0.05）. Conclusion Large dose of VitD3 could lead to the serious calcification located in media of aorta in mouse, and the vascular smooth muscle cell coverted to analogical chondro-cyte. The mechanism was connected to that large dose of VitD3 which induces the convertion of vascular smooth muscle cell to analogical chondrocyte and simultaneously leads to the massive deposition of Ca and P in blood vessel.